Method for detecting monocytelike myeloid-derived suppressor cells in human peripheral blood
A technology for inhibiting cells and detection methods, applied in measuring devices, biological testing, material inspection products, etc., can solve problems such as the detection method of mo-MDSCs not given, and achieve easy widespread popularization, convenient operation, and scientific and credible methods Effect
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[0043] Example 1 The steps and conditions of a method for detecting mononuclear myeloid-derived suppressor cells in human peripheral blood are as follows:
[0044] 1. Antibodies used
[0045] (101) Antibody APC anti-human CD33, CD33 for short; BD Biosciences, USA;
[0046] (102) Antibody PE anti-human CD15, CD15 for short; BD Biosciences, USA;
[0047] (103) Antibody PE-CF594 anti-human HLA-DR, HLA-DR for short; BD Biosciences, USA;
[0048] (104) Antibody APC-H7 anti-human CD14, CD14 for short; BD Biosciences, USA;
[0049] (105) Antibody PE-Cy7 anti-human CD45, CD45 for short; BD Biosciences, USA;
[0050] (106) Antibody FITC anti-mouse CD11b, referred to as CD11b; Tianjin Sanjian Biotechnology Co., Ltd.
[0051] 2. Preparation of the solution used
[0052] (201) Preparation of PBS solution: weigh 8 g of NaCl, 0.2 g of KCl, NaCl 2 HPO 4 1.44 g and KH 2 PO 4 Dissolve 0.24 g in 1 L of ultrapure water, autoclave to obtain a PBS solution, and store in a refrigerator a...
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