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Thin-layer sample application method for preparing thin-layer chromatography

A thin-layer chromatography and spotting technology, applied in the field of thin-layer spotting, can solve problems such as difficult analysis, sample separation and preparation, and band crossing, and achieve the effects of facilitating sample development, fewer steps, and easy promotion.

Inactive Publication Date: 2017-08-22
LIAONING UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In the conventional preparation method, when spotting samples on the preparation plate, dot-like spotting is commonly used, and wavy bands appear on the thin-layer plate. In this way, for samples with poor separation effect, the bands of multiple compounds may cross, which is difficult to analyze and sample preparation

Method used

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  • Thin-layer sample application method for preparing thin-layer chromatography
  • Thin-layer sample application method for preparing thin-layer chromatography
  • Thin-layer sample application method for preparing thin-layer chromatography

Examples

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Embodiment 1

[0025] The Radix Paeoniae Alba extract is obtained by a method in the prior art.

[0026] 1) Preparation of TLC

[0027] Weigh 5 g of the binder carboxymethylcellulose sodium (CMC-Na) into a beaker, add 1 L of distilled water, heat and stir on an electric stove for about 30 min until completely dissolved, cool, and prepare 0.5% CMC aqueous solution for later use. Weigh 40g of silica gel and 70mL of 0.5% CMC aqueous solution, mix well, stir continuously, let stand, stir again until all the silica gel is completely wetted, and finally use ultrasonic treatment for about 4-5min to fully discharge the bubbles in the solution. Use a horn spoon to pour the prepared silica gel onto a 20cm×20cm square glass plate, spread it evenly with a horn spoon, and lightly shake it on the table until the surface is flat and smooth. After drying at room temperature, put it in an oven and heat at 105° C. for 30 minutes to prepare a thin-layer plate 5 , put it in a desiccator, and store it.

[0028...

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Abstract

The invention discloses a thin-layer sampling method for preparing thin-layer chromatography. The invention belongs to the technical field of thin-layer chromatography analysis and preparation. The method of the invention mainly includes the following steps: preparation of thin layer plate, preparation of sample pointing device, sample pointing, sample development and sample enrichment. The method of the invention has the advantages of simple equipment, easy operation, quickness, convenience, low cost and good separation effect after sample unfolding.

Description

technical field [0001] The invention belongs to the technical field of thin-layer chromatography analysis and preparation, and in particular relates to a method for preparing thin-layer sample. Background technique [0002] Thin-layer chromatography has simple equipment, convenient operation, wide adaptability, and can quickly give reliable and accurate experimental results. It is an alternative economical, reliable and rapid analysis method. Commonly used thin-layer chromatography has a small amount of sample load, and is mostly used for qualitative analysis, and cannot prepare a large number of samples. Preparative chromatography is an extension of the application of commonly used thin-layer chromatography, which overcomes the shortcomings of commonly used thin-layer chromatography. The high-efficiency separation effect can also achieve the effect of preparation on the mixture, and has the advantages of convenient operation, simple equipment, and fast analysis and preparat...

Claims

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Application Information

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IPC IPC(8): G01N30/90G01N30/91
CPCG01N30/90G01N30/91
Inventor 刘玉峰孙珊珊朱丽君胡延喜徐亮桑育黎卢晓丹
Owner LIAONING UNIVERSITY
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