Gas dynamic mixing device and method for cell culture

Abstract

The invention discloses a gas dynamic mixing device and method for cell culture in the field of gas mixing and detection. An iron core in a gas mixing chamber drives a left bracket and a movable thin plate to move left and right, a mixed gas flows from the bottom to the top through a gas vent to the top of the gas mixing chamber, a CO2 sensor detects CO2 concentration, an MCU controller calculates concentration difference between two successive tests, if the concentration difference is less than a set threshold value, the mixed gas is output; otherwise, the mixed gas in the gas mixing chamber is introduced into the gas mixing chamber to mix again through a recycle gas pipeline pump. According to the gas dynamic mixing device and method for the cell culture, the switch-on and switch-off of an electromagnet and the action of a spring are utilized to move the thin plate of the gas vent back and forth, gas molecules constantly hit the thin plate to change paths and hit against each other, the purpose of full and rapid mixing of the gas is achieved, the dynamic mixing and detection of the gas is completed, and according to the feedback of the CO2 sensor, the gas inside the gas mixing chamber is controlled to be discharged or conduct a second mixing to achieve the purpose of feedback regulation.

Description

technical field [0001] The invention relates to the field of gas mixing and detection, which enables dynamic mixing of two gases and CO 2 Mixed with air for cell culture. Background technique [0002] In the fields of cell culture and medical equipment, gas mixing devices are generally used to uniformly mix different gases before use. Existing gas mixing devices are limited to static mixing of gases. Two gases are introduced into a container at the same time, and the movement path of the gas is guided by the shape of the mixing pipe for two or more mixings, and then through the diffusion between the gases There are many problems in this gas mixing method, for example, the mixing is not enough and uneven, the mixing effect is not good, and the mixing speed is slow. When used in cell culture, CO 2 Mixes with air to form CO 2 For the mixed gas with a concentration of 5%, the existing gas mixing device also has the problem of poor mixing effect. Contents of the invention ...

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Problems solved by technology

[0003] The purpose of the present invention is to solve the problems existing in the existing gas mixing device, and propose a gas dynamic mixing device for cell culture and the mixing method of the device, which can better make two different gases mix quickly and uniformly , fully mixed

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