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Major gene TaMYC1 for controlling the character of purple seed coat in common wheat and application of the gene

A main effect gene and gene technology, applied in the field of TaMYC1 gene, the main effect gene controlling purple seed coat traits, can solve the problem that the function of TaMYC1 has not been clearly verified

Inactive Publication Date: 2017-07-14
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Pp3 TaMYC1 is speculated to encode the bHLH gene (Di et al., 2016; Shoeva et al., 2014), but the function of TaMYC1 has not been clearly verified

Method used

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  • Major gene TaMYC1 for controlling the character of purple seed coat in common wheat and application of the gene
  • Major gene TaMYC1 for controlling the character of purple seed coat in common wheat and application of the gene
  • Major gene TaMYC1 for controlling the character of purple seed coat in common wheat and application of the gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 The main gene TaMYC1 gene controlling purple seed coat traits in common wheat is derived from the bHLH transcription factor TaMYC1 of wheat varieties Gaoyuan 115 and Opata, and it has the first to 4584th positions of SEQ ID NO.1 in the sequence table Nucleotide sequence shown.

[0039] The TaMYC1 gene includes at least 6 transcript genes; wherein the TaMYC1-1 gene has the nucleotide sequence shown in the 1st to 1566th position of SEQ ID NO.2 in the sequence listing; the TaMYC1-2 gene has the SEQ ID NO.3 in the sequence listing The nucleotide sequence shown in the 1st to 1635th positions; the TaMYC1-3 gene has the nucleotide sequence shown in the 1st to 1707th positions of SEQ ID NO.4 in the sequence listing; the TaMYC1-4 gene has the SEQ ID NO.4 in the sequence listing. The nucleotide sequence shown in the 1st to 1741th positions of ID NO.5; the TaMYC1-5 gene has the nucleotide sequence shown in the 1st to 1743rd positions of SEQ ID NO.6 in the sequence listin...

Embodiment 2

[0133] Example 2 The TaMYC1 gene, the main gene controlling purple seed coat traits in common wheat, includes the TaMYC1 gene isolated from wheat varieties Gaoyuan 115 and Opata as a part of the recombinant vector, which is used as a part of the host cell.

Embodiment 3

[0134] Example 3 The TaMYC1 gene, the main gene controlling purple seed coat traits in common wheat, includes the TaMYC1 gene isolated from wheat varieties Gaoyuan 115 and Opata as a part of the host cell.

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PUM

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Abstract

The invention relates to a major gene TaMYC1 for controlling the character of purple seed coat in common wheat, wherein the gene is sourced from a bHLH transcription factor TaMYC1 from the wheat varieties of Highland 115 and Opata. The gene has the nucleotide sequence represented as the 1st-4584th loca in the sequence table represented as the SEQ ID No.1. The gene can be applied to cultivation of a transfection plant.

Description

technical field [0001] The invention relates to the field of plant genetic engineering, in particular to the main gene TaMYC1 gene controlling purple seed coat traits in common wheat and its application. Background technique [0002] Anthocyanins are a class of secondary metabolites synthesized by most plants. They are responsible for flower and fruit pigmentation and can attract pollen and seed dispersal vectors (Joaquin-Cruz et al., 2015). In vegetative tissues, they are involved in the development and protection of nonspecific disease resistance in plants in response to stress from biotic and abiotic factors (Treutter, 2006). Environmental conditions such as high light intensity, low temperature, high salinity or drought stress, can affect anthocyanin production (Chalker-Scott and Tinnemore, 2009). Anthocyanins have gained attention due to their anti-inflammatory, antimutagenic, anticancer and antimicrobial properties as well as roles in hepatotoxicity and induction of ...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/82A01H5/10
CPCC07K14/415C12N15/825
Inventor 刘宝龙王道文张怀刚席杏媛宗渊陈文杰张波魏乐
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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