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A method for rapid and directional acquisition of young nymphs

A technology of Aphid spp. and farnesene is applied in the field of insect biology, which can solve the problems of inability to distinguish winged or wingless, inability to compare the differential expression changes of genes in the critical period of wing type differentiation, etc., and achieves convenient operation and wide application. Foreground effect

Active Publication Date: 2020-03-27
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current research has found that the pseudo-embryos of A. sativa, the 1st and 2nd instar nymphs cannot be distinguished from winged or wingless by appearance. Swell and grow wing buds to distinguish between winged and wingless aphids
Therefore, current studies on the molecular mechanism of aphid wing type differentiation are all aimed at gene expression analysis after aphid wing type differentiation, and it is impossible to compare the differential expression changes of genes in the critical period of wing type differentiation

Method used

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  • A method for rapid and directional acquisition of young nymphs
  • A method for rapid and directional acquisition of young nymphs
  • A method for rapid and directional acquisition of young nymphs

Examples

Experimental program
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Embodiment

[0024] S1: Select 10 adult aphids of wingless wheat aphid and cultivate them separately in a petri dish for three generations. In each generation, select 3 healthy aphids and continue to cultivate them separately;

[0025] S2: Raise the fourth generation of aphids individually to the 3rd instar stage, and then take 10 of them and place them in the same closed petri dish with a diameter of 120mm;

[0026] S3: Add 2μL of E-β-farnesene at a concentration of 500ng / μL into a closed petri dish, the solvent of the E-β-farnesene solution is n-hexane, and then add once every 2h, adding 5 times as a cycle ; Repeat step S301 for 4 cycles (ie, a total of 5 days of treatment); among them, the time of the first addition of E-β-farnesene solution in each cycle is the same as the time of the first addition of E-β-farnesene solution in the first cycle Point, during this period, remove all the aphids produced by adult aphids;

[0027] After adding the E-β-farnesene solution for 5 days, collect the yo...

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Abstract

The invention relates to a method for obtaining young winged aphids of macrosiphum avenae in a rapid and directional mode. The method comprises the steps that S1, adult wingless aphids of macrosiphum avenae are raised in a single mode, and propagation of three generation is carried out; S2, aphids of the fourth generation are raised to third instar in a single mode, and a plurality of raised aphids of the fourth generation are put into a same culture device; S3, E-beta-farnesene solutions are added into the culture device repeatedly, and after the E-beta-farnesene solutions are added, young winged pseudo embryos and / or aphids of macrosiphum avenae are collected. According to the method for obtaining the young winged aphids of macrosiphum avenae in a directional mode, pseudo embryos which can develop winged macrosiphum avenae, first instar aphids and second instar aphids which can develop winged macrosiphum avenae, third instar aphids of winged macrosiphum avenae, fourth instar aphids of winged macrosiphum avenae and adult aphids of winged macrosiphum avenae are obtained in a directional mode, and the basis of the aphid gene expression study of the pseudo embryo period, first instar period and second instar period is provided; the method is convenient, simple and easy to operate, and wide in application prospect.

Description

Technical field [0001] The invention relates to the technical field of insect biology, in particular to a method for quickly and directionally acquiring the young winged aphid of the tube aphid. Background technique [0002] The wheat aphid has a typical proliferation polymorphism. Under the regulation of external environmental factors, a single genetic type of wheat aphid can produce two different biotypes during development: winged and wingless. Like other winged dimorphic insects, the winged wheat aphid can carry out long-distance migratory dispersal. After reaching the destination, it can form a wingless aphid for reproduction and expansion, and the large-scale reproduction of the offspring is harmful. Developmental plasticity is an important survival strategy formed by aphids in the long-term evolutionary process to adapt to the ever-changing ecological environment. If the formation of winged aphids can be effectively prevented, the food loss caused by wheat aphids will be s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 李祥瑞张方梅张云慧魏长平程登发朱勋
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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