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Plant differential metabolite fast-screening method based on UPLC-QTOF

A screening method and differential technology, applied in the field of rapid screening of plant differential metabolites based on UPLC-QTOF, can solve problems such as incomplete chromatographic extraction, increased workload, and reduced analysis efficiency

Active Publication Date: 2017-06-13
NINGXIA MEDICAL UNIV
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Problems solved by technology

However, the XCMS method has the problem of incomplete chromatographic extraction
In addition, the current method generally has the problem of false positives, requiring human intervention, adding additional workload and reducing analysis efficiency

Method used

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  • Plant differential metabolite fast-screening method based on UPLC-QTOF
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  • Plant differential metabolite fast-screening method based on UPLC-QTOF

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Embodiment Construction

[0038] In combination with the accompanying drawings of the present invention, the technical solutions of the embodiments of the invention will be further elaborated in detail.

[0039] The samples were three groups of the same plant species but different years. All samples were processed as follows:

[0040] A method for rapid screening of plant differential metabolites based on UPLC-QTOF, comprising the following steps:

[0041] S1: Sample preparation process: put freshly collected tea samples into liquid nitrogen for quick freezing, grind and pulverize the samples under liquid nitrogen conditions, add 2ml of extract to 20mg of pulverized samples, the extract includes 3 parts by volume of acetonitrile, 3 parts by volume of isopropanol and 2 parts by volume of water, vortexed for 2 minutes, ultrasonicated at room temperature for 60 minutes, then centrifuged at 12000r / min for 5 minutes, and 1ml of the supernatant was transferred to a chromatographic vial for UPLC-QTOF analysi...

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Abstract

The invention provides a plant differential metabolite fast-screening method based on UPLC-QTOF. The method comprises acquiring information of chemical constituents in the plant through an UPLC-QTOF modern analytical technology, extracting substance information through a non-target metabolic profile analysis technology, carrying out chromatographic peak extraction and in-source pyrolysis molecular fragment clustering, carrying out between-sample time drifting correction through a dynamic planning method, carrying out between-sample substance registration according to a self-adaptive network link method and screening sample different metabolites through statistical analysis.

Description

technical field [0001] The invention relates to a method for analyzing chemical components contained in a sample, in particular to a method for rapidly screening plant differential metabolites based on UPLC-QTOF. Background technique [0002] At present, in the field of quality control of plant extracts such as traditional Chinese medicine, flavors and fragrances, obtaining methods that can characterize the differences between sample groups such as different origins and batches, and based on this to control the quality of products is not only an industrial enterprise, but also a scientific research. The unit is very interested in the field. However, since the quality of plant extracts is the overall result of the metabolites produced by the interaction between the plant and the growth environment, the differences in the external macroscopic quality are mostly due to the differences in the composition of some components and samples from other groups. How to screen these diff...

Claims

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Application Information

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IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8809
Inventor 于永杰付海燕佘远斌
Owner NINGXIA MEDICAL UNIV
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