A high-efficiency extraction and purification method of Sclerotin and its application

Abstract

The invention discloses an efficient scleroglucan extraction and purificatiodn method and an application thereof. The method includes: 1), performing microwave enhanced dissolution after dilute alkali dissolution by a mycelium contained scleroglucan crude product or a high-concentration scleroglucan fermentation liquid raw material; 2), performing separation on the scleroglucan solution obtained form the step 1) to remove the insoluble mycelium with frame filter or centrifugal separation and merging a scrubbing solution into the scleroglucan solution by adopting the mycelium obtained from the scrubbing solution; 3), neutralizing the scleroglucan solution after bacterial removal; 4), treating the scleroglucan solution through an ultrafiltration membrane prior to desalting and concentrating, and washing the scleroglucan concentrated solution is washed through water and prior to desalting; 5), precipitating and washing the scleroglucan with ethyl alcohol to obtain the product after drying. Through treatment of a series of physical methods, the stable and efficient scleroglucan preparation process is established, chemical reaction is not needed, impurity components in the finished product are reduced, and the purity of the targeted scleroglucan product is up to 90%.

Description

technical field [0001] The invention relates to a high-efficiency extraction and purification method and application of microbial polysaccharide Sclerotinia polysaccharide, belonging to the technical field of microbial polysaccharide preparation. Background technique [0002] Scleroglucan, also known as scleroglucan or scleroglucan, is a water-soluble non-ionic exopolysaccharide synthesized and secreted by the filamentous fungus Sclerotinia. Its aqueous solution has good rheological properties and stability. Scleroglucan molecules are composed of linear β-D-(1,3)-glucose residue chains containing β-D-(1,6)-glucose residue side chains, with a molecular weight of about 5 to 6×10 6 Da, the molecule exists in the form of a rod-shaped triple helix, which is semi-rigid. Scleroglucan aqueous solution has high temperature resistance (up to 120°C), high salt resistance (up to 20,0000mg / L), acid and alkali resistance (stable within the pH range of 2~12), high shear resistance, good ...

AI Technical Summary

Problems solved by technology

In addition, the patent CN102154408A also reported the technical route of online membrane separation and extraction when the concentration of scleroglucan was low in the early stage of fermentation production, but the process was complicated and the production cost remained high

There are also some biological enzymatic hydrolysis pretreatment methods to purify scleroglucan, the cost of biological enzymes is high, and the temperature of the enzyme reaction needs to be controlled, and the reaction time is longer, especially for the treatment of high-viscosity solutions of Sclerotin, and the reaction mixing time is longer Long, and the introduction of enzyme impurities, the cost is high, these conditions are not conducive to industrial production

The purification methods in the prior art have all affected the development of scleroglucan industry and market applications