MrgprX2 high-expression reconstituted cell and MrgprX2 high-expression membrane receptor stationary phase and preparation method and application thereof

Abstract

The invention discloses an MrgprX2 high-expression reconstituted cell and MrgprX2 high-expression membrane receptor stationary phase and a preparation method and application thereof. The reconstituted cell uses HEK293 cells as host cells for transfecting expression carriers containing MrgprX2 full-length genes; the MrgPrX2 receptor macromolecules can be stably expressed on the cell membrane; in addition, the complete receptor biological activity is realized. The MrgprX2 high-expression membrane receptor stationary phase is manufactured by adsorbing cytomembranes of MrgprX2 high-expression reconstituted cells by activated silica gel; the application to the specific screening and identification of MrgprX2 triggered anaphylactoid ingredients can be realized. Through practical application, the condition that the sinomenine hydrochloride can be specifically combined with MrgprX2 is discovered. Meanwhile, the cell level allergic experiments prove that the sinomenine hydrochloride can stimulate mastocytes to release histamine and beta-hexosaminidase; the result proves that the MrgprX2 high-expression membrane receptor stationary phase can really be applied to the specific identification of the anaphylactoid ingredients.

Description

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AI Technical Summary

Benefits of technology

This patented technology allows creation of highly expressed cloned yeasts called Xm23a1 through introducing foreign genes or proteins involved in their metabolism. These introduced sequences have been shown to increase production rates during fermentation processes compared to previous methods. By adding these exogenous materials onto certain types of substrate like CHO cells they become more effective targets for developing new treatments against inflammations associated diseases caused by chemical substances used therein. Additionally, the use of special plasma reservoir systems provides efficient ways to isolate small amounts of active ingredients within them without losing any significant effectiveness over long periods of storage due to its ability to maintain conformation even when stored under different conditions. Overall, this innovative approach simplifies the manufacturing process and reduces costs per sample.

Problems solved by technology

The technical problem addressed in this patented study relates to finding out if certain chemicals or substances called lithargeletanes exist naturally within plants without being associated with harmful effects such as those caused by other natural products like pollen grains.

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