Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Lactobacillus paracasei n1115 culture medium and its application

A technology of Lactobacillus and para-cheese, applied in the field of bioengineering, can solve problems such as difficulties in the production of bacterial powder, achieve the effects of saving material and labor costs, simple configuration, and simple ingredients

Active Publication Date: 2020-06-16
正阳君乐宝乳业有限公司
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, my country's probiotics mainly rely on imports, and the production of domestic probiotic powder is difficult due to the existence of technical barriers
One of the big problems is the high-density cultivation of probiotics

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lactobacillus paracasei n1115 culture medium and its application
  • Lactobacillus paracasei n1115 culture medium and its application
  • Lactobacillus paracasei n1115 culture medium and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-5

[0031] Embodiment 1-5 discloses a kind of Lactobacillus paracasei N1115 culture medium, and the raw material content of each embodiment is as shown in table 1 below:

[0032] Table 1 Raw material content table

[0033]

[0034] The method for cultivating Lactobacillus paracasei N1115 in the substratum of application embodiment 1-5 is as follows:

[0035] A, preparation of bacterium suspension: after Lactobacillus paracasei N1115 is activated for two generations, streak is cultivated on the solid MRS medium plate, cultivated for 24h at 37°C, and then cultivated for 24h at 37°C in MRS medium to obtain a concentration of 10 6 cfu / mL bacterial suspension.

[0036] b. Fermentation: Inoculate the above bacterial suspension into the Lactobacillus paracasei N1115 culture medium of Example 1-5 with an inoculum amount of 1%, and culture at 37° C. for 24 hours.

[0037] Further, the process of step a activating two generations is as follows:

[0038] Lactobacillus paracasei N1115 w...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses Lactobacillus paracasei N1115 culture medium and also discloses a high-density culture method of Lactobacillus paracasei N1115. The Lactobacillus paracasei N1115 culture medium and its culture method have the advantages of good composition simplicity, simple configuration and low cost, material and manpower costs are saved, the concentration of live bacteria in fermentation broth acquired by culturing Lactobacillus paracasei N1115 with the Lactobacillus paracasei N1115 culture medium is 9.6*1010 cfu / mL.

Description

technical field [0001] The invention belongs to the field of bioengineering, and relates to a culture medium, in particular to a culture medium of Lactobacillus paracasei N1115, and at the same time, the invention also relates to a method for cultivating Lactobacillus paracasei N1115 at high density. Background technique [0002] At present, my country's probiotics mainly rely on imports. Due to the existence of technical barriers, the production of domestic probiotics powder is very difficult. One of the big problems is the high-density cultivation of probiotics. Research on high-density culture includes research on medium composition and culture conditions. [0003] The main components of the medium include: carbon source, nitrogen source, water, inorganic salts and growth factors. Carbon and nitrogen sources provide microorganisms with energy and the carbon and nitrogen elements needed to form themselves; water provides a stable growth environment for microorganisms; in...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/245
CPCC12N1/20
Inventor 王世杰魏立华朱宏冯丽莉荀一萍张栋郝红伟薛玉玲
Owner 正阳君乐宝乳业有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com