A microbial remediation method for oil-contaminated soil

A technology for microbial remediation and oil pollution. It is applied in the restoration of contaminated soil and biological sludge treatment. It can solve the problems of large pollution area, difficult treatment and high toxicity.

Inactive Publication Date: 2019-06-25
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is characterized by a large polluted area and complex composition of sludge, including water, petroleum, heavy metals, clay, sand and gravel, etc. It is difficult to treat and highly toxic

Method used

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  • A microbial remediation method for oil-contaminated soil
  • A microbial remediation method for oil-contaminated soil
  • A microbial remediation method for oil-contaminated soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] a. Collect oil-contaminated soil near the oil well, dry it and crush it to remove volatile hydrocarbons and water;

[0020] b. Add the biomass powder with a particle size of 1mm to the soil of step a by volume ratio of 10% as cotton stalk powder and mix evenly, and adjust the water content to 20% and adjust the pH to 7.5;

[0021] c. Preparation of crude surfactant: The inorganic salt medium is NH 4 Cl 0.776g, KH 2 PO 4 0.05g, K 2 HPO 4 0.05g, CaCl 2 0.001g, MgSO 4 0.01g, 100mL ultrapure water, pH 7.5, put into a 250mL Erlenmeyer flask, and add 4g n-hexadecane, sterilize at 121℃ for 20min, after cooling, insert Pseudomonasaeruginosa liquid, and use ventilation Seal the membrane and place it in a constant temperature shaker at a temperature of 30°C and incubate at 120r / min for 96 hours to obtain a crude surfactant with a surface tension of 26.68mN / m;

[0022] d. Mix Pseudomonas aeruginosa and Achromobacter sp at a volume ratio of 0.9:1, and connect the mixed bacteria liquid ...

Embodiment 2

[0025] a. Collect oil-contaminated soil near the oil well, dry it and crush it to remove volatile hydrocarbons and water;

[0026] b. Add biomass powder with a particle size of 2mm to the soil of step a by volume at 20% to mix evenly with sawdust, adjust the moisture content to 25%, and adjust the pH to 7.5;

[0027] c. Preparation of crude surfactant: The inorganic salt medium is NH 4 Cl 0.776g, KH 2 PO 4 0.05g, K 2 HPO 4 0.05g, CaCl 2 0.001g, MgSO 4 0.01g, 100mL ultrapure water, pH 7.5, put into a 250mL Erlenmeyer flask, and add 4g n-hexadecane, sterilize at 121℃ for 20min, after cooling, insert Pseudomonasaeruginosa liquid, and use ventilation Seal the membrane and place it in a constant temperature shaker at a temperature of 30°C and incubate at 120r / min for 96 hours to obtain a crude surfactant with a surface tension of 26.68mN / m;

[0028] d. Mix Pseudomonas aeruginosa and Achromobacter sp at a volume ratio of 0.9:1, and connect the mixed bacteria liquid to the soil system ob...

Embodiment 3

[0031] a. Collect oil-contaminated soil near the oil well, dry it and crush it to remove volatile hydrocarbons and water;

[0032] b. Add the biomass powder with a particle size of 3mm to the soil of step a by volume ratio 30% to mix evenly with rice husk, adjust the moisture content to 30%, and adjust the pH to 7.5;

[0033] c. Preparation of crude surfactant: The inorganic salt medium is NH 4 Cl 0.776g, KH 2 PO 4 0.05g, K 2 HPO 4 0.05g, CaCl 2 0.001g, MgSO 4 0.01g, 100mL ultrapure water, pH 7.5, put into a 250mL Erlenmeyer flask, and add 4g n-hexadecane, sterilize at 121℃ for 20min, after cooling, insert Pseudomonasaeruginosa liquid, and use ventilation Seal the membrane and place it in a constant temperature shaker at a temperature of 30°C and incubate at 120r / min for 96 hours to obtain a crude surfactant with a surface tension of 26.68mN / m;

[0034] d. Mix Pseudomonas aeruginosa and Achromobacter sp at a volume ratio of 0.9:1, and connect the mixed bacteria liquid to the soil s...

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Abstract

The invention relates to a microbial remediation method for petroleum-contaminated soil. The microbial remediation method is characterized in that pseudomonas aeruginosa, achromobacter sp and a biosurfactant crude product are combined to repair petroleum-contaminated soil or degrade petroleum in oil sludge. The method has remarkable degradation effect on petroleum-contaminated soil or petroleum hydrocarbon in the oil sludge. According to a test, initial oil content of the petroleum-contaminated soil is 20000 mg / kg to 130000 mg / kg, and degradation efficiency can reach 75-91%. The method has the advantages of being low in treatment cost, high in treatment efficiency, free of secondary pollution and the like.

Description

Technical field [0001] The invention belongs to a method for remediating petroleum-contaminated soil and treating oil sludge, and specifically relates to a microbial remediation method for petroleum-contaminated soil in arid areas in northwestern China. Background technique [0002] my country is a big country of oil production and consumption. Due to the damage and leakage of oil storage tanks, oil gathering pipelines and underground storage tanks of petroleum and petrochemical enterprises and the occurrence of emergencies during drilling and production, the surrounding soil has been polluted to a certain extent. It is characterized by large polluted area and complex composition of sludge, including water, petroleum, heavy metals, clay, sand, etc., and its treatment is difficult and highly toxic. At present, the domestic and foreign treatment methods for sludge are: solvent extraction method, hot lotion method, solidification method, pyrolysis method, biological method (in-situ,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B09C1/10C02F11/02
CPCB09C1/10C02F11/02
Inventor 贾汉忠马健波王传义屈撑囤
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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