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A Micro-Size Measurement Method Based on a Randomly Reconfigurable Micro-Size Measuring Device Based on Magnetic Fluorescent Microspheres

A technology of fluorescent microspheres and measuring devices, which is applied in the direction of measuring devices, optical devices, instruments, etc., can solve the problems of large probe size, limited application range, and measurement dependence, so as to improve the measurement speed and expand the measurement application range , the effect of avoiding errors

Active Publication Date: 2019-01-22
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The disadvantages of the existing probes described in the above documents are: (1) the production of the probe is difficult and the size of the measuring head is large, and the application range is limited; (2) the measurement is limited by the depth-to-diameter ratio of the workpiece to be measured and the probe Natural frequency limitation; (3) measurement depends on the load of high-precision motion mechanism

Method used

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  • A Micro-Size Measurement Method Based on a Randomly Reconfigurable Micro-Size Measuring Device Based on Magnetic Fluorescent Microspheres
  • A Micro-Size Measurement Method Based on a Randomly Reconfigurable Micro-Size Measuring Device Based on Magnetic Fluorescent Microspheres
  • A Micro-Size Measurement Method Based on a Randomly Reconfigurable Micro-Size Measuring Device Based on Magnetic Fluorescent Microspheres

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specific Embodiment approach 1

[0021] Specific implementation mode one: combine Figure 1 to Figure 3 To illustrate this embodiment, the random reconfiguration micro-size measurement device based on magnetic fluorescent microspheres described in this embodiment includes: a dichroic mirror 2, an objective lens 3, a sample chamber 5, two layers of controllable magnetic poles 7, a collection lens 9 and CCD10;

[0022] Along the optical axis, a CCD10, a collection lens 9, a dichroic mirror 2, an objective lens 3 and a sample chamber 5 are arranged sequentially from top to bottom;

[0023] The excitation light beam 1 hits the dichroic mirror 2, and after being reflected by the dichroic mirror 2, it enters the sample chamber 5 through the objective lens 3, and the sample chamber 5 contains a solution containing a large amount of magnetic fluorescent microspheres 6.

[0024] Two layers of controllable magnetic poles 7 are aligned up and down in parallel and evenly arranged around the sample chamber 5,

[0025] T...

specific Embodiment approach 2

[0032] Specific embodiment two: this embodiment is based on the micro-size measurement method of the device described in embodiment one, the method is:

[0033] The part of the sample 4 to be measured is immersed in a solution containing magnetic fluorescent microspheres 6, the light intensity of the excitation light beam 1 is adjusted, and the excitation light beam 1 is irradiated to the dichroic mirror 2, so that the excitation light beam 1 passes through The objective lens 3 can irradiate the magnetic fluorescent microsphere 6, and make it emit a fluorescent beam 8, and randomly change the magnetic field strength and magnetic field direction of the two layers of controllable magnetic poles 7 many times, so that a randomly changing magnetic field is generated in the sample chamber 5 to control the magnetic fluorescence. The microsphere 6 moves randomly in the solution;

[0034] During the change of the magnetic field, the CCD10 is used to collect multiple images of fluoresce...

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Abstract

A random reconstruction micro-dimension measuring device based on magnetic fluorescent microspheres and a micro-dimension measuring method thereof belong to the field of industrial sample micro-dimension measurement and aim to settle defects in prior art. The micro-dimension measuring method comprises the steps of transmitting an excited light beam to a dichroic lens so that the excited light beam can be irradiated to magnetic fluorescent microspheres through an objective lens; the magnetic fluorescent microspheres transmit fluorescent light for randomly changing magnetic field strength and magnetic field direction of a two-layer controllable magnetic pole for multiple times; a randomly changed magnetic field is generated in a sample room; and the magnetic fluorescent microspheres are randomly moved in solution. A CCD is utilized for acquiring multiple fluorescent light spot images at different time points. Positions of the magnetic fluorescent microspheres in the axial direction of an optical axis are acquired according to light strength distribution of the light spots. The positions of the magnetic fluorescent microspheres in the radial direction of the optical axis are acquired according to the positions of the light spots. Image reconstruction is performed for acquiring sample information and finishing dimension measurement of a measured sample. The random reconstruction micro-dimension measuring device and the micro-dimension measuring method thereof are suitable for fields of chemistry and biomedicine.

Description

technical field [0001] The invention belongs to the field of micro-size measurement of industrial samples, and mainly relates to a random reconfiguration micro-size measurement method based on magnetic fluorescent microspheres. Background technique [0002] At present, magnetic fluorescent microspheres are mainly used in the fields of chemistry and biomedicine, and are often used in the purification of biological samples, cell separation, and single-molecule force spectroscopy. However, the present invention applies magnetic fluorescent microspheres to three-dimensional measurement of the size of industrial samples. [0003] At present, the most effective three-dimensional measurement method for micro-sized industrial samples is to use a combination of a miniature three-coordinate measuring machine and a contact probe sensor system for measurement. The measurement principle of this measurement method is that the external motion mechanism drives the probe to contact the teste...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01B11/00G01B11/24
CPCG01B11/00G01B11/24
Inventor 倪赫邹丽敏张鹏丁雪梅
Owner HARBIN INST OF TECH
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