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Inducible type promoter and application thereof

A promoter and inducible technology, applied in the field of plant genetic engineering

Active Publication Date: 2017-05-10
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the transcriptional regulation mechanism of the agarwood sesquiterpene synthase gene has not been reported yet

Method used

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  • Inducible type promoter and application thereof
  • Inducible type promoter and application thereof
  • Inducible type promoter and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] (1) Expression vector construction

[0058] Using the genomic DNA of A. chinensis as a template, PCR amplification was carried out with specific primers, and four different promoter fragments were obtained. Four different promoter fragments were digested with SacI and SpeI respectively, and the digested products were ligated with the pAN580 (containing green fluorescent protein GFP) vector fragment obtained through the same digestion to obtain the ligated product pAN580-ASS1P1-GFP ( Marked as GFP1), pAN580-ASS1P2-GFP (marked as GFP2), pAN580-ASS1P3-GFP (marked as GFP3), pAN580-ASS1P4-GFP (marked as GFP41), the four constructs were transformed into Escherichia coli respectively. The extracted transformant plasmid was digested and sequenced to ensure correct construction.

[0059] Wherein, the promoter sequence of GFP1 is the sequence shown in the 1-973bp region of the nucleotide sequence shown in SEQ ID NO.2.

[0060] The promoter sequence of GFP2 is the sequence shown...

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Abstract

The invention relates to an inducible type promoter, and further relates to an expression vector containing the inducible type promoter and application of the inducible type promoter in regulating the expression of a target gene. The inducible type promoter has a function of starting the expression of a downstream structural protein gene under an induction condition. A technical support is provided to utilize the inducible expression mechanism of the promoter to improve the synthesis expression amount of aquilaria sinensis sesquiterpenes, and understand the mechanism related to trauma inducible expression of an aquilaria sinensis sesquiterpenes synthase gene.

Description

technical field [0001] The present disclosure relates to the field of plant genetic engineering, in particular to an inducible promoter and its application. Background technique [0002] Aquilaria sinensis (Aquilaria sinensis) is a rare and endangered plant unique to my country, and it is the only authentic plant source for the production of precious aromatic medicinal materials Aquilaria sinensis. Studies have shown that sesquiterpenoids are the most important components of Agarwood medicinal materials and the main components of their essential oils (Hashimoto et al., 1985; Chen et al., 2011, Chen et al., 2012). Agarwood sesquiterpenes are almost absent in healthy A. argentina, and are synthesized by the corresponding metabolic pathway after the tree is induced by injury. [0003] Sesquiterpene synthase is a key enzyme in the sesquiterpene biosynthesis pathway that catalyzes the synthesis of sesquiterpene from FPP. The sesquiterpene synthase gene ASS1 is a typical induced...

Claims

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Application Information

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IPC IPC(8): C12N15/113C12N15/82A01H5/00
CPCC07K14/415C12N15/8243
Inventor 魏建和徐艳红孙佩文吕菲菲高志晖廖永翠张争杨云金钺
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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