Method for detecting disinfection liquid residue on explant surface during tissue culture

Abstract

The embodiments of the present invention disclose a method for detecting the disinfection liquid residue on the explant surface during tissue culture. The method comprises: washing: washing explant surface to obtain a washed explant; disinfecting: soaking the washed explant in 70% alcohol and a 5-10% NaClO solution; disinfecting liquid residue removing: rinsing the disinfected explant by using sterile water; and detecting: adding a cresol red indicator to the last sterile water washing waste liquid in a dropwise manner, and observing the color of the waste liquid to determine the residue. According to the present invention, the washing agent is rationally used in the explant disinfecting method , and the explant is subjected to a series of the disinfecting operations and the detection step by matching the cleaning, detecting and observing technology; and the detection method is simple and easy to perform, does not cause any added damage to the explant, can accurately determine the disinfecting liquid residue removing degree, and improves the production efficiency under the premise of the ensuring of the explant survival rate.

Description

technical field [0001] The invention relates to the technical field of tissue culture, in particular to a method for detecting residual disinfectant on the surface of explants in tissue culture. Background technique [0002] Most of the explant materials used for plant tissue culture are taken from the field, but because a large number of microorganisms are attached to the plant surface. Therefore, disinfection treatment must be carried out before plant material inoculation, the purpose is to kill various microorganisms on the surface of the material without affecting its growth. [0003] In order to prevent the damage to explants caused by the residual disinfectant, it is necessary to wash the explants with sterile water to remove the residual disinfectant after disinfection. However, since the general disinfectant is colorless, the removal of the residual is not easy to observe with the naked eye, so it will Incomplete removal of residues will affect the survival rate of ...

AI Technical Summary

Problems solved by technology

[0003] In order to prevent the damage to explants caused by the residual disinfectant, it is necessary to wash the explants with sterile water to remove the residual disinfectant after disinfection. However, since the general disinfectant is colorless, the removal of the residual is not easy to observe with the naked eye, so it will Incomplete removal of residues will affect the survival rate of explants or excessive flushing of explants will reduce production efficiency