Method for purifying aescin from semen aesculi extract

A kind of technology of aescin and sage, applied in the direction of organic chemistry, steroids, etc., can solve the problems of unable to meet the production requirements of industrialization and controllability, uncontrollable implementation operation, and high requirements for radiation equipment

Inactive Publication Date: 2017-04-05
XIAN BOTANICAL GARDEN SHAANXI PROV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The technical effect of this new technology was that it improved upon previous methods by improving efficiency while reducing costs compared to existing techniques.

Problems solved by technology

Technologies described in this patents involve various techniques like preparations containing salixselenidine with improved properties including better effectiveness against inflammation caused by tissue damage, reduced side effects associated with traditional drugs, lower costs compared to current therapies, increased safety during long term storage, minimal environmental impact on manufacturing processes, and enhanced bioavailability through prolonged release over several hours without excessive exposure.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Preparation of Sala Seed Extract

[0029] Harvest the mature Sala seeds of Aescinus sinensis, dry the raw Sala seeds, crush them through a 40-mesh sieve, and set aside. Take by weighing 500g of powdered salsa seed raw material, add 8 times the amount of 65% ethanol (ethanol volume consumption is 4L) to extract overnight, and constantly stir, ultrasonically assisted extraction 3 times under the condition of 26 ° C, the ultrasonic extraction frequency is 25KHz, stir Make it in a suspended state, ultrasonic extraction time is 0.5h each time, combine the extracts and centrifuge. Concentrate under reduced pressure in a water bath, the degree of vacuum is less than or equal to 0.095Mpa, and the heating temperature of the water bath is 50°C to obtain 128.3g of the extract of Sala japonica, with a yield of 25.6%.

[0030] (2) Acetone precipitation

[0031] Add 0.5L of methanol to the above-mentioned extract of saffron seeds, and dissolve the extract of saffron seeds with ...

Embodiment 2

[0037] (1) Preparation of Sala Seed Extract

[0038] The preparation of the fruit extract is the same as that described in Example 1, except that the fruit used comes from Zhejiang Aesculus. Take by weighing 1000g powdery salsa seed raw material, add 8 times the amount of 65% ethanol (ethanol volume consumption is 8L) leaching overnight, and constantly stir, under the condition of 30 ℃, ultrasonic assisted extraction 3 times, ultrasonic extraction frequency is 25KHz, stir Make it in a suspended state, ultrasonic extraction time is 0.5h each time, combine the extracts and centrifuge. Concentrate under reduced pressure to obtain 254.7 g of the extract extract of Sala seed, with a yield of 25.5%.

[0039] (2) Acetone precipitation

[0040]Add 4 times the amount (1L) of methanol or ethanol to 254.7g of the extract of saffron seeds, and dissolve the extract completely, then add 1L of acetone, stir to make it in a suspended state, and set it aside for 3 hours at 16°C , the yellow...

Embodiment 3

[0046] (1) Preparation of Sala Seed Extract

[0047] The preparation of the sala seed extract is the same as that described in Example 1, except that the sala seed used comes from Tianshi chestnut. Take by weighing 2000g powdery salsa seed raw material, add 6 times the amount of 65% ethanol (volume of ethanol is 12L) to extract overnight, and keep stirring, ultrasonically assisted extraction 3 times under the condition of 28 ℃, ultrasonic extraction frequency is 25KHz, stir Make it in a suspended state, ultrasonic extraction time is 0.5h each time, combine the extracts and centrifuge. Concentrate under reduced pressure to obtain 502.4 g of the extract of Sala seed extract, with a yield of 25.1%.

[0048] (2) Acetone precipitation

[0049] Add 3 times the amount (1.5L) of methanol to 502.4g of the extract of the scorpion extract, and dissolve the extract completely, then add 1.5L of acetone, stir to make it in a suspended state, and set it aside for 2 hours at 20°C , the yel...

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PUM

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Abstract

The invention discloses a method for purifying aescin from a semen aesculi extract. The method can comprise the steps that (1) sedimentation is conducted through acetone, specifically, the semen aesculi extract is dissolved through 3-5 times amount of low mass molecule alcohol, the acetone of the equal volume is added in a suspended status, stewing and sedimentation are conducted at 15-20 DEG C, and yellowish-brown sediment on the lower layer is obtained, decompressed and concentrated to obtain an aescin extract; (2) purification is conducted through macroporous adsorption resin, specifically, the extract is completely dissolved through 1-2 times amount of pure water, adsorption is conducted through the macroporous adsorption resin AB-8 with the sample loading quantity being 5g of the extract per 100 ml of the resin and the flow rate being 0.1-0.3 BV/h, elution is conducted through ethyl alcohol with the elution speed controlled to be 0.5-1 BV/h, and aescin flow is collected; and (3) washing and decoloration are conducted through acetone, specifically, the aescin flow is decompressed and concentrated, and obtained power is washed and decolored through the acetone, and subjected to vacuum drying to obtain an off-white aescin product. By adoption of the method, the aescin can be purified from the semen aesculi extract, and the method has the advantages that technological operation is easy and convenient, and the method is efficient and low in energy consumption, and meets production requirements of large-scale industrial extraction and purification of the aescin.

Description

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Claims

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Application Information

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Owner XIAN BOTANICAL GARDEN SHAANXI PROV
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