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Method of inducing rooting of Quercus acutissima Carruth tissue culture seedlings

A technology of Quercus variegata and Quercus variegata, which is applied in the field of rooting induction of Quercus variegata tissue culture seedlings, can solve the problems of uneven rooting, not thick root system, and small number of roots, so as to achieve more roots, shorten the rooting cycle, and fast rooting speed Effect

Inactive Publication Date: 2017-03-08
LIUZHOU LINGTONG TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology improves plant material by optimizing certain factors like nutrients for better healthy plants or reducing harmful substances released when they grow new crops. By doing this with modifying media containing different chemical compounds that help it germinate faster and stronger roots, researchers aim to create consistently superior crop varieties through breeding techniques such as genetic engineering methods. Additionally, these modifications enhance stem cell activity and increase resistance against stressors while also promoting their ability to form adventitious roots called rootlets. Overall, this innovative process allows scientists to improve the production efficiency of various agricultural products without compromising beneficial properties.

Problems solved by technology

This patented describes different ways to grow quacrum carries out research into its properties such as growth rates, rootings, soil conditions, nutrients absorption ability, environmental impacts, etc., which affect their potential use value.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Select the secondary buds of Quercus japonica that have been cultured for 35-36 days in the conventional tissue culture, and after disinfecting the surface of the bottle, in the aseptic space on the ultra-clean workbench, select the vigorous growth of the secondary bud clusters with a height of 1-2 cm. Single buds, cut at 2-3mm below the node, and remove the base leaves and petioles. The pruned single buds were inoculated in the pre-rooting medium, and placed under the conditions of temperature 20±1°C, humidity 40%, light intensity 2000lux, and light 16h / d for pre-rooting culture. The raw material content of the pre-rooting medium described therein is: 1 / 2 improved MS medium+NAA 1.0mg / L+vitamin C6g / L++VC 15mg / L+sucrose 25g / L+agar 5.0g / L.

[0023] After 30-35 days of pre-rooting culture for single buds, transfer the single buds to the rooting medium and place them under the conditions of temperature 20±1°C, humidity 40%, light intensity 2000lux, and light 16h / d for rooti...

Embodiment 2

[0026] Select the secondary buds of Quercus japonica that have been cultured for 36-37 days in the conventional tissue culture, and after disinfecting the surface of the bottle, in the aseptic space on the ultra-clean workbench, select the vigorous growth of the secondary bud clusters, with a height of 1-2 cm. Single bud, cut at 2-3mm below the node, and remove the base leaves and petioles. The pruned single buds were inoculated in the pre-rooting medium, and placed under the conditions of temperature 20±1°C, humidity 40%, light intensity 2500lux, and light 15h / d for pre-rooting culture. The raw material content of the pre-rooting medium described therein is: 1 / 2 improved MS medium+NAA 1.5mg / L+vitamin C6g / L+VC 15mg / L+sucrose 25g / L+agar 5.0g / L.

[0027] After 30-35 days of pre-rooting culture for single buds, transfer the single buds into the rooting medium and place them under the conditions of temperature 20±1°C, humidity 40%, light intensity 2500lux, and light 15h / d for root...

Embodiment 3

[0030] Select the secondary buds of Quercus japonica that have been cultured for 37-38 days in the conventional tissue culture, and after disinfecting the surface of the bottle, in the sterile space on the ultra-clean workbench, select the secondary buds that grow robustly and have a height of 1-2 cm. Single buds, cut at 2-3mm below the node, and remove the base leaves and petioles. The pruned single buds were inoculated in the pre-rooting medium, and placed under the conditions of temperature 20±1°C, humidity 45%, light intensity 2000lux, and light 16h / d for pre-rooting culture. The raw material content of the pre-rooting medium described therein is: 1 / 2 improved MS medium+NAA 2.0mg / L+vitamin C6g / L++VC 15mg / L+sucrose 25g / L+agar 5.0g / L.

[0031] After 30-35 days of pre-rooting culture for single buds, transfer the single buds into the rooting medium, and place them under the conditions of temperature 20±1°C, humidity 45%, light intensity 2000lux, and light 16h / d for rooting cu...

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PUM

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Abstract

The invention discloses a method for inducing rooting of Quercus acutissima Carruth tissue culture seedlings. The method includes the steps of conducting bud strengthening culture of Quercus acutissima Carruth subculture buds in a conventional culture medium for 35-40 days, conducting pruning, then inoculating the buds in a pre-rooting culture medium, conducting pre-rooting culture for 30-35 days, and transferring the buds into a rooting culture medium for culture. According to the method, the rooting cycle of the Quercus acutissima Carruth subculture buds is shortened, the rooting rate is high, the Quercus acutissima Carruth has many root systems and high quality, the transplanting survival rate of the rooting tissue culture seedlings is high, the industrialization of the Quercus acutissima Carruth tissue culture seedlings can be achieved, and high quality seedlings are provided for artificial clonal forest construction; the method has good economic benefits, social benefits and ecological benefits.

Description

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Claims

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Application Information

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Owner LIUZHOU LINGTONG TECH CO LTD
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