A kind of plant lactobacillus derived from giant panda and its application

A technology of Lactobacillus plantarum and giant panda, applied in bacteria, microorganisms, microorganisms, etc., can solve problems such as transient immune protection, and achieve the effects of improving the expression of intestinal tight junction-related proteins, protecting intestinal damage, and reducing gene content.

Inactive Publication Date: 2019-11-08
SICHUAN AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, probiotic adhesion is host species-specific, and the use of heterologous probiotics may only exert transient immune protection

Method used

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  • A kind of plant lactobacillus derived from giant panda and its application
  • A kind of plant lactobacillus derived from giant panda and its application
  • A kind of plant lactobacillus derived from giant panda and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The isolation and identification of embodiment 1 bacteria

[0030] 1. Isolation of bacteria

[0031] Weigh 10g of the feces inside the fresh giant panda feces ball, add it into a triangular flask (with glass beads) filled with 90mL sterile normal saline, oscillate and mix for 20min, and serially dilute 10 times to 10 -3 sample liquid. Take 100 μL10 -3 The dilution sample solution was evenly coated on the MRS plate, and after anaerobic culture in a 37°C constant temperature incubator for 24-48 hours, a single colony of suspected lactic acid bacteria was picked up by the inoculation loop and streaked and purified repeatedly on the MRS plate until no bacteria were detected under the microscope.

[0032] 2. Identification

[0033] (1) According to the method described in "Berger Bacteria Identification Handbook" (Eighth Edition) and "Lactic Acid Bacteria Isolation and Identification and Experimental Methods" (edited by Ling Daiwen, Dong Xiuzhu, etc., Beijing: China Light...

Embodiment 2

[0050] Example 2 Effect of Lactobacillus plantarum BSGP201683 on the immune response and intestinal flora of mice infected with Escherichia coli

[0051] 1.1 Animal grouping and handling

[0052] 90 normal 4-week-old female ICR mice (16±2g) were randomly divided into 5 groups (n=18) after one week of adaptive feeding: blank group, enterotoxigenic Escherichia coli infection group, and low-dose Lactobacillus group , medium-dose Lactobacillus group and high-dose Lactobacillus group, the test period was 21 days. From day 1 to 14 of the experiment, mice in the blank control group and enterotoxigenic Escherichia coli infection group were fed with 200 μL of sterile PBS per day, and the mice in the low-, medium-, and high-dose Lactobacillus groups were fed with 200 μL of 5.0×10 8 CFU / mL, 5.0×10 9 CFU / mL and 5.0×10 10 CFU / mL L.plantarum BSGP201683 bacterial suspension. On the 15th day of the test, the blank group was still fed with sterile PBS, and the mice in the other test groups...

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Abstract

This application discloses a giant panda-derived plant lactobacillus and its application. The strain was preserved in the China Center for Type Culture Collection on May 4, 2016, and the preservation number is CCTCC NO: M2016245. The giant panda-derived plant lactobacillus provided by the present invention can increase the levels of serum IgA, IgM, and IgG immunoglobulin antibodies, inhibit the decrease in the percentage of lymphocytes and the increase in the percentage of neutrophils induced by enterotoxigenic Escherichia coli infection; significantly down-regulate inflammation-related The mRNA expression levels of factors IL‑1β, IL‑8, IL‑6, TLR4 and MyD88 alleviate the intestinal acute inflammatory response caused by enterotoxigenic Escherichia coli.

Description

technical field [0001] The application belongs to the field of biotechnology, and in particular relates to a giant panda-derived plant lactobacillus and its application. Background technique [0002] In the past few decades, people have devoted themselves to the improvement of giant panda nutrition and reproductive technology, ignoring the prevention and control of infectious gastrointestinal diseases. Enterotoxigenic Escherichia coli (ETEC) is one of the common pathogens of gastrointestinal diseases in captive giant pandas. mortality rate. Currently, the control of ETEC infection and its associated diarrhea largely relies on antibiotics, however, the emergence of drug-resistant strains and multi-drug resistance poses a potential threat to public health. Seeking economically viable alternatives to antibiotics such as organic acids, probiotics or prebiotics for the control of gastrointestinal infectious diseases is a current research hotspot. [0003] Probiotics are live m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/747A61P31/04C12R1/25
CPCA61K2035/115C12N1/205C12R2001/25
Inventor 曾东王强彭芝榕倪学勤牛李丽潘康成景波刘倩赖婧周毅
Owner SICHUAN AGRI UNIV
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