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Coating and modification method for prussian blue nanometer mesocrystal cytomembrane

A technology of Prussian blue and modification method, which is applied in the field of drug preparation, can solve the problems of poor biofilm coating effect, short blood circulation time, immune system clearance, etc., and achieve the effect of good modification effect, short time consumption, and evasion of phagocytosis

Inactive Publication Date: 2017-02-01
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the short blood circulation time of Prussian blue nanomaterials in biological applications, it is easy to be cleared by the immune system; the existing nanoparticle biofilm coating effect is poor, the surface modification of nanoparticles is required, the equipment is expensive, and the production efficiency is low. Problem: The present invention provides a Prussian blue nano-mesogenic cell membrane coating modification method; it aims to improve the potential and application effect of Prussian blue nano-materials in biological applications by means of biofilm coating and modification

Method used

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  • Coating and modification method for prussian blue nanometer mesocrystal cytomembrane
  • Coating and modification method for prussian blue nanometer mesocrystal cytomembrane
  • Coating and modification method for prussian blue nanometer mesocrystal cytomembrane

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] HPB coated with erythrocyte membrane:

[0052] Step (1): Take by weighing 3g polyvinylpyrrolidone (PVP-K30) and 132mg potassium ferricyanide (K 3 [Fe(CN) 6 ]·3H 2 (2) Dissolve with a hydrochloric acid solution with a volume of 40mL and an HCl concentration of 0.01M, stir at room temperature for 30min to obtain a clear yellow-green solution, stop stirring, heat the solution to 80°C for 20-24h, stop heating until the solution drops to room temperature , 12000r / min centrifugal washing, to obtain solid Prussian blue nano-mesocrystalline solid (MPB).

[0053] Step (2): Add a polytetrafluoroethylene liner and add a hydrochloric acid solution with a volume of 20mL and a HCl concentration of 1.0M, and then add MPB (20mg) and PVP (100mg) prepared in step (1) under magnetic stirring , after stirring at room temperature for 3 hours, transfer to the reactor and heat up to 140°C for 4 hours, stop the reaction, centrifuge and wash at 12000r / min, freeze-dry to obtain dark blue holl...

Embodiment 2

[0059] Preparation method of hollow Prussian blue nano-mesogens loaded with drug-modified erythrocyte membrane:

[0060] Step (1): Prepare hollow Prussian blue nano-mesogens and RBC suspension according to the method described in Example 1. Disperse 1 mg of HPB evenly in a solution of doxorubicin hydrochloride with a concentration of 10 mg / mL, and let it stand overnight; then centrifuge at 10,000 r / min to remove free doxorubicin hydrochloride to obtain HPB loaded with doxorubicin hydrochloride;

[0061] Step (2): Turn on the ultrasound (ultrasonic parameters are the same as in Example 1), and the RBC solution (the PBS solution of RBC, the mass content of RBC is 1wt%) with a pH of 7.4 is quickly added to the centrifuged obtained loaded doxorubicin hydrochloride (RBC / HPB dosing weight ratio 1:1), continue to sonicate until the mixed solution is clear, and then centrifuge to remove excess RBC, and then obtain DOX-loaded hollow Prussian blue nano-mesogens modified by erythrocyte m...

Embodiment 3

[0066] Compared with Example 2, the difference is that the doxorubicin hydrochloride is replaced by FITC-labeled cytochrome C (marked as FITClabeledCytochrome C). Prepared hollow Prussian blue nanocrystals loaded with FITC labeled Cytochrome C (marked as RBC@FITC labeled Cytochrome C-HPB.)

[0067] The photothermal stimulation release test was carried out on the prepared RBC@FITC labeled cytochrome C-HPB:

[0068] Take 1.5mL of RBC@FITC labeled cytochrome C-HPB with a concentration of 2mg / mL in a 3mL glass bottle, and use a power of 1.0W / cm 2 The 808nm fiber-optic coupled continuous semiconductor laser light irradiated the bottom of the glass bottle, and 100 μL was taken at 5, 10, 15, 20, and 25 minutes to test the fluorescence intensity of FITC labeled on cytochrome c to determine the release amount of cytochrome c. When the same concentration of RBC@FITC labeled Cytochrome C-HPB was not illuminated under the same conditions, the released amount of cytochrome c was measured ...

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Abstract

The invention discloses a coating and modification method for a prussian blue nanometer mesocrystal cytomembrane. The method comprises the steps that biological membrane and prussian blue nanometer mesocrystal are coated with ultrasonic waves at the pH of 7.4-8.0, and then the prussian blue nanometer mesocrystal coated with the biological membrane is obtained through centrifugalization, wherein the ultrasonic power is 150-300 w, and the ultrasonic frequency is 20-40 kHz. In the coating and modification method, the biological membrane and the prussian blue nanometer mesocrystal are coated efficiently by the coating and modification method with ultrasonic waves at the pH. Moreover, according to the method, the hydrophobic treatment or other pretreatments of the prussian blue nanometer mesocrystal are not needed, the operation is simple, the coating effects are good, and after the prussian blue nanometer mesocrystal is coated and modified, the effects of the prussian blue in the body are improved greatly.

Description

technical field [0001] The invention belongs to the field of medicine preparation, and in particular relates to a method for coating and modifying Prussian blue nano-mesogenic cell membranes. Background technique [0002] Prussian blue has been used as a dye for 200 years. Prussian blue nanomaterials have been found to have good application prospects in photophysics, electricity, optics and other fields, and have been widely used in electrochemistry, catalysis, analysis and other fields. Prussian blue nanomaterials have the advantages of simple preparation process, mild reaction conditions, low cost, stable chemical structure, etc., and have been certified by the US Food and Drug Administration (FDA) in 2003. Recently, scientists discovered that Prussian blue has strong light absorption in the near-infrared region, has high photothermal conversion efficiency, and can be used as an MRI contrast agent, which has been widely used in the diagnosis and treatment of diseases. [...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K9/50A61K45/06A61K47/46A61K49/18
Inventor 刘又年曾科陈万松欧阳江刘颖刘洪邓留阳明辉
Owner CENT SOUTH UNIV
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