Microbial fermentation feed and preparation method and application thereof
A technology of microbial fermentation and compound microbial bacteria, applied in animal feed, animal feed, application, etc., can solve the problems of low cellulose degradation rate, low fermentation efficiency, poor process, etc., achieve high protein content, improve survival rate, Good for growth and development
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Embodiment 1
[0058] (1) Mix the plant-based material corn stalks and cakes (a mixture of soybean meal and rapeseed meal, the mass ratio is 1:1) according to the mass ratio of 5:1 and then pulverize, then add the compound enzyme preparation (cellulase, dextran enzyme, papain and pectinase) mixed evenly, and hydrolyzed under the conditions of pH 4.5 and temperature 35°C, wherein the solid-liquid ratio was 1:6, cellulase, dextranase, papain The consumption of pectinase and pectinase is respectively 400u / g plant base material, 200u / g plant base material, 200u / g plant base material, 200u / g plant base material; obtain enzymatic hydrolysis product;
[0059] (2) Mix the enzymatic hydrolyzate prepared in step (1) with the rumen content taken out from the rumen in the fresh cow stomach, wherein the mass ratio of the plant base material to the rumen content is 200:1; Anaerobic liquid fermentation for 12 hours under the condition of ℃, drying at 35 ℃, crushing and passing through a 60-mesh sieve to ob...
Embodiment 2
[0068] (1) The plant-based material corn stalks and cakes (a mixture of soybean meal and rapeseed meal, the mass ratio is 1:3) are mixed in a mass ratio of 2:1 and then pulverized, and then compound enzyme preparations (cellulase, dextran enzyme, papain and pectinase) were mixed evenly, and hydrolyzed at pH 4 and temperature 37°C, wherein the solid-liquid ratio was 1:5, cellulase, dextranase, papain The consumption of pectinase and pectinase is respectively 350u / g plant base material, 180u / g plant base material, 170u / g plant base material, 150u / g plant base material; obtain enzymatic hydrolysis product;
[0069] (2) Mix the enzymatic hydrolyzate prepared in step (1) with the rumen content taken out from the rumen in the fresh cow stomach, wherein the mass ratio of the plant base material to the rumen content is 150:1; Anaerobic liquid fermentation under the condition of 18h, dried at 45°C, crushed and passed through a 20-mesh sieve to obtain the fermented product 1;
[0070] ...
Embodiment 3
[0078] (1) The plant-based material corn stalks, cake meal (a mixture of soybean meal and rapeseed meal, the mass ratio is 1:2) and bran are mixed according to the mass ratio of 1:1:1 and then pulverized, and then the compound enzyme preparation (cellulose enzyme, dextranase, papain and pectinase) were mixed evenly, and hydrolyzed under the conditions of pH 5 and temperature 40°C, wherein the solid-liquid ratio was 1:3, cellulase, glucan The dosages of carbohydrase, papain and pectinase are 300u / g plant base material, 150u / g plant base material, 150u / g plant base material, 180u / g plant base material respectively; enzymatic hydrolysis products are obtained;
[0079] (2) Mix the enzymatic hydrolyzate prepared in step (1) with the rumen content taken out from the rumen in the fresh cow stomach, wherein the mass ratio of the plant base material to the rumen content is 100:1; Anaerobic liquid fermentation for 20 hours under the condition of ℃, drying at 37 ℃, crushing and passing t...
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