Identification method for nitrogen-containing compound in Biluochun green tea
A nitrogen compound and identification method technology, applied in the field of analysis and detection, can solve the problems of inaccurate composition and concentration of nitrogen-containing compounds, and achieve the effect of meeting the needs of rapid detection in production, wide applicability, and simple operation
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[0020] This method mainly uses headspace solid-phase microextraction combined with gas chromatography-nitrogen and phosphorus detector-mass spectrometry to quickly detect and qualitatively quantify nitrogen-containing compounds in Biluochun green tea. The specific steps are as follows:
[0021] 1) Extract nitrogen-containing compounds in Biluochun green tea: 1) Weigh 80g of deionized water into a 250ml Erlenmeyer flask, heat the deionized water to 80°C for later use; 2) Accurately weigh 80g of commercially available Biluochun green tea, pour the tea sample into into the deionized water prepared in step 1), shake well and soak. 3) Soaking takes 10 minutes in total, and the conical flask needs to be shaken every 2 minutes during the process to shake the tea leaves evenly. 4) After soaking is completed, filter through a sieve, and then rapidly cool the filtered tea soup to room temperature. 5) Take 5g of cooled tea soup, add 1g of sodium chloride and 20μL of 2-methoxy-3-methylpy...
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