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Application of soybean transcription factor GmbZIP336 and soybean transcription factor GmbZIP336 encoding gene in regulation of grain weight of seed

A transgenic plant, grain weight technology, applied in the application, genetic engineering, plant genetic improvement and other directions, can solve the problems of backwardness and inability to meet the needs of the people

Active Publication Date: 2016-12-07
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Soybean production lags far behind the pace of development of other domestic food crops and cannot meet national needs

Method used

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  • Application of soybean transcription factor GmbZIP336 and soybean transcription factor GmbZIP336 encoding gene in regulation of grain weight of seed
  • Application of soybean transcription factor GmbZIP336 and soybean transcription factor GmbZIP336 encoding gene in regulation of grain weight of seed
  • Application of soybean transcription factor GmbZIP336 and soybean transcription factor GmbZIP336 encoding gene in regulation of grain weight of seed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] Example 1, Expression Analysis of GmbZIP336 Gene in Different Developmental Processes of Soybean

[0088] 1. Taking the weight of soybean Williams 82 seeds as a standard (the specific standard is the weight percentage of developing seeds accounting for plump but not yet dehydrated seeds), the soybean development process is divided into 7 stages, and the weight percentages corresponding to the 7 stages are 4%, 8%, 12%, 16%, 24%, 48%, 96% ( figure 1 ). The transcriptomes of the two stages of seed development stage 3 (12%) and 5 (24%) were sequenced and compared, and transcription factors highly expressed in stage 6 were obtained, including Glyma13g39340. After comparison with the database, the It was named GmbZIP336 gene.

[0089] 2. Extract the RNA of each developmental stage of soybean seed development, reverse transcribe to obtain cDNA; use cDNA as template, use F and R to carry out Real Time-PCR, detect the expression level of GmbZIP336 gene, use soybean Tublin gene...

Embodiment 2

[0095] Embodiment 2, the acquisition of transgenic GmbZIP336 Arabidopsis and its phenotype analysis

[0096] 1. Using Gateway technology to construct overexpression vectors

[0097] 1. Acquisition of GmbZIP336 gene

[0098] The total RNA of soybean Williams 82 seedlings was extracted and reverse transcribed to obtain cDNA; the cDNA was used as a template and GmbZIP336-up and GmbZIP336-dp were used as primers for PCR amplification to obtain the GmbZIP336 gene. The primer sequences are as follows:

[0099] GmbZIP336-up: 5'-GGGGACAAGTTTGTACAAAAAAGCAGGCTTCATGTCTCTCCAAACAACCAAATCAA-3' (SEQ ID NO: 3);

[0100] GmbZIP336-dp: 5'-GGGGACCACTTTGTACAAGAAAGCTGGGTTCCAGGATGCGCTTAGAGGCCTCC-3' (SEQ ID NO: 4).

[0101] 2. Press TA The kit (Invitogen product, catalog number 12536-017) instruction manual comes with the operation steps to get the GmbZIP336 gene and entry vector obtained above 8 / GW / TOPO( TA (included in the kit) for the BP recombination reaction to obtain the BP reacti...

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Abstract

The invention discloses an application of a soybean transcription factor GmbZIP336 and a soybean transcription factor GmbZIP336 encoding gene in the regulation of the grain weight of a seed. The thousand-seed weight of transgenic Arabidopis thaliana grains obtained by transfering GmbZIP336 gene to wild Arabidopis thaliana is substantially higher than that of wild Arabidopis thaliana, so a GmbZIP336 protein has an up-regulation effect on the grain weight of the seed, and over-expression of the GmbZIP336 encoding gene improves the seed weight of transgenic genes without influencing the normal growth of the plants. Experiments prove that the GmbZIP336 protein can be used as a target gene for improving the seed output of the plants, and has important application values in the field of plant breeding.

Description

technical field [0001] The invention relates to the application of soybean transcription factor GmbZIP336 and its encoding gene in regulating seed weight, and belongs to the field of biotechnology. Background technique [0002] Soybean is an important traditional crop with rich nutritional value. It is an important economic crop to provide grain, oil and feed. Soybean oil also has many uses in industrial production, such as biofuel, surfactant, softener, etc. China used to be the world's largest soybean producer. However, in recent years, my country's soybean production has only accounted for one-third of the demand, making China the world's largest soybean importer, with total imports accounting for half of the world's total soybean exports. Soybean production lags far behind the pace of development of other domestic food crops and cannot meet national needs. In the past 50 years, soybean production has only increased by 62%, while other food crops have increased by more t...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/82C12N5/10C12N15/11A01H5/00
Inventor 张劲松陈受宜陆翔马彪张万科林晴何锶洁
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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