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A detection method for gadolinium-containing hydroxyapatite nanoparticles

A technology containing gadolinium hydroxyapatite and gadolinium hydroxyapatite, which is applied to the analysis of materials and instruments, can solve the problems of inaccurate detection, systematic error, and inability to reflect the original characteristics of nano-HA-Gd biological effects, etc. Achieve good light stability and high detection accuracy

Active Publication Date: 2020-08-07
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method has the following two main defects: first, the physical and chemical properties of nano-HA-Gd labeled with fluorescent substances will change, for example, the active functional groups on the surface are occupied by fluorescent compounds, the surface charge changes, and the particle size changes. The interaction with cells is different from the effect of nano-HA-Gd not labeled with fluorescence, so it cannot reflect the most real and original characteristics of the biological effect of nano-HA-Gd, thus affecting the nano-HA-Gd to be tested Judgment of biological effects; second, there is the problem of photobleaching: since the fluorescence intensity of most fluorescent substances will be significantly weakened after long-term and continuous laser irradiation, they cannot be accurately detected under CLSM for a long time, so imaging, quantitative system error is inevitable

Method used

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  • A detection method for gadolinium-containing hydroxyapatite nanoparticles
  • A detection method for gadolinium-containing hydroxyapatite nanoparticles
  • A detection method for gadolinium-containing hydroxyapatite nanoparticles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1: Dried nano-HA-Gd is detected and scanned by CLSM.

[0049] Suspend a small amount of nano-HA-Gd in a small amount of absolute ethanol, drop it into a CLSM glass dish, wait for the absolute ethanol to evaporate, and place it under a CLSM microscope. During the spectral scanning process, the excitation wavelength can be set to any one or several excitation wavelengths within the range of 200nm to 1050nm, and the detection wavelength range can be set to the detection wavelength range including the excitation wavelength within the range of 200nm to 1050nm. In this embodiment, lasers with four wavelengths are selected: UV-405nm, Ar-488nm, Kr-561nm, HeNe-633nm, the detection wavelength range is set to 400nm to 650nm, and the step distance is 2nm, and the light intensity-wavelength curve is drawn.

[0050] Test results such as figure 1 Shown in (a) is the emission spectrum of nano-HA-Gd in the receiving wavelength range of 400-650nm. There is a sharp peak at 405, ...

Embodiment 2

[0051] Example 2: Dried nano-HA-Gd-FITC was detected and scanned by CLSM.

[0052] Suspend a small amount of nano-HA-Gd-FITC in a small amount of absolute ethanol, drop it into a CLSM glass dish, wait for the absolute ethanol to evaporate, and place it under a CLSM microscope. FITC solution was used as the control group. During the spectral scanning process, the excitation wavelength can be set to any one or several excitation wavelengths within the range of 200nm to 1050nm, including the excitation wavelength of FITC, and the detection wavelength range can be set to within the range of 200nm to 1050nm, including the excitation wavelength and FITC The detection wavelength range of the detection wavelength. In this embodiment, lasers with four wavelengths are selected: UV-405nm, Ar-488nm, Kr-561nm, HeNe-633nm, the detection wavelength range is set to 400nm to 650nm, and the step distance is 2nm, and the light intensity-wavelength curve is drawn.

[0053] Test results such as ...

Embodiment 3

[0056] Example 3: Quantitative analysis of dried nano-HA-Gd.

[0057] 1. Operation steps:

[0058] 1.1. Dilute nano-HA-Gd with ethanol step by step, add a drop of ethanol suspension to the CLSM glass dish in turn, wait for the ethanol to volatilize, use the reflected light channel under CLSM (with a group of reflected light of excitation light) For example, excitation at 561nm, detection of reflected light at 561nm) to image it; extract the light signal area from the image by an image analyzer, combine the corresponding concentration ratio, and draw a standard curve;

[0059] 1.2. Dilute the analyte containing nano-HA-Gd with ethanol, add a drop of suspended ethanol solution to the CLSM glass dish, wait for the ethanol to volatilize, and use the reflected light channel to image it under CLSM; use the image analyzer to analyze the image Extract the light signal area and correspond to the standard curve to obtain the concentration of nano-HA-Gd in the analyte.

[0060] The opt...

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Abstract

The invention provides a detection method of gadolinium-containing hydroxyapatite nanoparticles. The detection method comprises steps as follows: a to-be-detected sample is prepared and contains the gadolinium-containing hydroxyapatite nanoparticles and / or fluorescently-labeled gadolinium-containing hydroxyapatite nanoparticles; the to-be-detected sample is radiated by laser, and reflection light is collected; an image or a spectral curve is formed according to the collected reflection light. By means of the method, continuous, long-time and real-time tracking and positioning imaging of the gadolinium-containing hydroxyapatite nanoparticles can be realized.

Description

technical field [0001] The invention relates to a detection method for gadolinium-containing hydroxyapatite nanoparticles, in particular to a laser imaging detection method for gadolinium-containing hydroxyapatite nanoparticles. Background technique [0002] Hydroxyapatite is similar to the inorganic components of human and animal bones and teeth, and has attracted extensive attention in various fields of biomaterials in recent years. Trivalent gadolinium ions (Gadolinium ions, Gd 3+ ) with and calcium ions (Ca 2+ ) similar bioinorganic chemical properties and coordination. Gd 3+ Able to influence the remodeling cycle of bones and have the potential to treat diseases such as osteoporosis. Hydroxyapatite and a certain amount of Gd 3+ Combined, the synthetic formula is Ca 10-x Gd x (PO 4 ) 6 (OH) y Gadolinium-containing hydroxyapatite is expected to optimize its biochemical properties. [0003] Gadolinium-containing hydroxyapatite acts on organisms mainly in the for...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N15/00
Inventor 袁兰黄健袁帅军
Owner PEKING UNIV
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