Molecular marker of loin-eye area character-related gene SVEP1 and application of molecular marker

Abstract

The invention relates to the field of preparation of a swine molecular marker, in particular to a molecular marker of a loin-eye area character-related gene SVEP1 and an application of the molecular marker. According to the molecular marker, the sequence of the SVEP1 gene comprises an SNP site which is located on the 44660bpth part of a nucleotide sequence shown as SEQ ID NO.1, and the site is an A / G base mutation which causes SfanI-RFLP polymorphism. The base mutation, which is located within the 3rd intron of the SVEP1 gene, cannot change an amino acid sequence which is translated by the base mutation. Therefore, a sequence, which is shown as SEQ ID NO.2, of the molecular marker can be obtained by designing a primer nearby the SNP site of the SVEP1 gene sequence, and subsequently, loin-eye area characters can be identified. According to the patent, on the basis of an existing PCR-RFLP technology, the A / G mutation is discovered on the 44660bpth part of the SVEP1 gene, and it discovers that the polymorphic site is significantly related to the loin-eye area characters; therefore, the patent is helpful for swine growth character researches to a certain degree.

Description

technical field [0001] The invention relates to the field of porcine molecular marker preparation, in particular to a molecular marker of eye muscle area trait-related gene SVEP1 and its application. Background technique [0002] Animal husbandry occupies a very important position in the development of modern agriculture, and its ratio in agricultural production is usually used as an important indicator to measure the development level of a country and region. Pork is the most important source of meat in China, and the pig breeding industry occupies a pivotal position in my country's animal husbandry. In the mid-1980s, my country’s meat output was about 20 million tons, and pork accounted for about 85%. In 2012, pork output reached 53.35 million tons, accounting for about 63.63% of the total meat. Although the proportion of pork has gradually decreased, it has always occupied a dominant position. [0003] With the development of society and the improvement of consumption l...

AI Technical Summary

Problems solved by technology

(Liu Xiaochun. Pig Molecular Breeding and Application Prospect. Porcine Science. 2011(07): 100-3.) Usually, the eye muscle area is usually measured by the slaughter method, but in large-scale breeding, this method not only Time-consuming and labor-intensive, and it can only measure post-mortem individuals, which has great limitations

Although A-ultrasound and B-ultrasound have certain applications in measuring the eye muscle area of ​​living pigs, the measurement results are easily affected by various aspects, such as: pigs cannot be completely stabilized, and are easily affected by the measurement environment and cause stress, etc. Therefore, The use of such methods in large-scale breeding work will introduce a large number of inaccurate factors, which will eventually affect the breeding effect (Yang Xiujuan, Deng Bin, etc. Research on live A-ultrasound measurement technology of pig backfat thickness and eye muscle thickness: [J]. Northwest Agriculture and Forestry Journal of University of Science and Technology, 2014, 42(5): 23-27; Liu Wei, Wu Haohao, etc. Uncertainty assessment of pig backfat thickness and eye muscle area in live pigs: [J]. Shanghai Animal Husbandry and Veterinary Communication, 2013, 6:77)

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