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A kind of Lactococcus gordonii and biological antiseptic preservative and application thereof

A technology of Lactococcus gordonii and bio-preservation, applied in the field of microorganisms, can solve problems such as residues, toxic chemicals, agricultural product quality and people's health effects, and achieve inhibition of respiratory intensity, reduction of water loss from tubers, and excellent antibacterial effect Effect

Active Publication Date: 2019-05-31
徐州双信食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, chemical agents are mainly used to prevent and control them. Chemical agents are toxic and have residues, which have an important impact on the quality of agricultural products and people's health.

Method used

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  • A kind of Lactococcus gordonii and biological antiseptic preservative and application thereof
  • A kind of Lactococcus gordonii and biological antiseptic preservative and application thereof
  • A kind of Lactococcus gordonii and biological antiseptic preservative and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Isolation and identification of Lactococcus gasseri SY-1

[0038] 1. Separation

[0039] Take 50g of fresh yam skin (with soil), smash it, and mix it evenly. Take 10g of the treated yam skin and pour it into a 250mL triangular flask containing 20 fine glass beads and 100mL sterilized deionized water, and set it on a shaker at 150r / min , shaken at 25°C for 1 hour, and after standing still for 30 minutes, take 1 mL of the supernatant and dilute it with 10-fold gradient dilution with sterilized deionized water to obtain 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 、10 -7Take 100 μL of each of the 7 gradients, apply different gradient dilutions on the ATB solid plate medium, and culture at 25-28°C for 30-36 hours. Use an inoculation loop to pick a single colony, streak and purify it on the ATB solid medium plate for 2-3 times, transfer the number of the purified single colony with different forms to the ATB solid plate, and culture it statically for 30-36 hours...

Embodiment 2

[0046] The application of embodiment 2 bacterial strains

[0047] 1. Antibacterial test

[0048] Preparation of Lactococcus gasseri SY-1 fermentation broth: take the preserved strain of Lactococcus gasseri SY-1, underline it on the ATB solid medium plate by aseptic operation, and carry out activation culture; after activation culture, pick Take a single colony and inoculate it into a 250mL Erlenmeyer flask containing 50mL of ATB liquid medium, and cultivate it to obtain a seed liquid; inoculate the seed liquid into a new ATB medium at a volume ratio of 5%, and culture it statically at 28°C for 24h , to obtain fermentation broth;

[0049] Preparation of Jerusalem artichoke leaf extract: take fresh Jerusalem artichoke leaves, grind, beat, mix according to 1:10 solid-liquid ratio (g / L) and place in 50% ethanol aqueous solution for ultrasonic extraction, extraction temperature is 40°C, extraction time is 60min, Ultrasonic power 800w; after extraction, under 8000g centrifugal for...

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Abstract

The invention discloses a Lactococcus garsneri, a biological preservative and an application thereof. The Lactococcus garseri is named as Lactococcus garviea SY-1, and the preservation number is CGMCC No.9485. The biological antiseptic preservative contains the fermentation liquid of the Lactococcus gasseri. The fermented liquid of Lactococcus grisneri SY-1 of the present invention has strong antibacterial and antibacterial activities, especially mixed with Jerusalem artichoke leaf extract in a certain proportion and used synergistically, and has a good control effect on black spot of root crops such as yams , greatly reducing its incidence rate, prolonging its storage time, and also has a certain freshness preservation effect; the mixture of Lactococcus grisneri SY-1 fermentation liquid and Jerusalem artichoke leaf extract of the present invention has safety, high efficiency, non-toxicity, no residue, It has many advantages such as being edible, and has good economic and social value.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a Lactococcus gasseri and a biological antiseptic and fresh-keeping agent and applications thereof. Background technique [0002] Lactic acid bacteria is a general term for a class of bacteria that can use fermentable carbohydrates to produce a large amount of lactic acid. It is widely distributed in nature and has rich species diversity. They have extremely high application value in fields closely related to human life such as industry, agriculture, food and medicine, so they have received great attention and attention from people. Bacteriocins are a class of polypeptides or precursor polypeptides with antibacterial activity produced by bacteria through the ribosome synthesis mechanism during metabolism. It presents a narrow inhibitory spectrum to the same kind of related strains. It perforates the target cells, inhibits the synthesis of peptidoglycan, interacts with rib...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23B7/155C12R1/01
CPCA23B7/155C12N1/205C12R2001/46
Inventor 马艳弘李亚辉殷剑美张宏志张培通黄开红
Owner 徐州双信食品有限公司
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