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Immune cell cryopreservation solution and application thereof

A technology of immune cells and cryopreservation solution, applied in the field of immune cell cryopreservation and cell cryopreservation, can solve the problems of cryopreserved cell survival rate and low cell activity, reduce the possibility of animal pathogen contamination and maintain cell stability , Improve the effect of activity stability

Active Publication Date: 2016-06-08
居李生物科技(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The advantage of using this freezing solution is that it will not introduce foreign proteins, which reduces the possibility of animal pathogen contamination. However, the survival rate and cell activity of frozen cells in this freezing solution are still low

Method used

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  • Immune cell cryopreservation solution and application thereof
  • Immune cell cryopreservation solution and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0077] Example 1: Preparation of Immune Cell Cryopreservation Solution (Recombinant Human Interleukin-2+Polyethylene Glycol+1,2-Propanediol+RPMI-1640 Medium+Human Serum Albumin)

[0078] According to volume content, 1.0g / ml human serum albumin, 100U / mL recombinant human interleukin-2, 0.1g / mL polyethylene glycol, 0.2g / mL 1,2-propanediol and 99% RPMI- The 1640 medium was mixed evenly to prepare the immune cell cryopreservation solution, which was stored at 4°C for later use.

Embodiment 2

[0079] Example 2: Preparation of Immune Cell Cryopreservation Solution (Recombinant Human Interleukin-2+Polyethylene Glycol+1,2-Propanediol+RPMI-1640 Medium+Human Serum Albumin)

[0080] According to volume content, 1.0g / ml human serum albumin, 50U / mL recombinant human interleukin-2, 0.15g / mL polyethylene glycol, 0.25g / mL 1,2-propanediol and 98% RPMI- The 1640 medium was mixed evenly to prepare the immune cell cryopreservation solution, which was stored at 4°C for later use.

Embodiment 3

[0081] Example 3: Preparation of Immune Cell Cryopreservation Solution (Recombinant Human Interleukin-2+Polyethylene Glycol+1,2-Propanediol+RPMI-1640 Medium+Human Serum Albumin)

[0082] According to volume content, 1.0g / ml human serum albumin, 200U / mL recombinant human interleukin-2, 0.2g / mL polyethylene glycol, 0.3g / mL 1,2-propanediol and 95% RPMI- The 1640 medium was mixed evenly to prepare the immune cell cryopreservation solution, which was stored at 4°C for later use.

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Abstract

The invention relates to an immune cell cryopreservation solution and application thereof. The immune cell cryopreservation solution is prepared from 250-200 U / mL of recombinant human interleukin-2, 0.1-0.4 g / mL of polyethylene glycol, 0.1-0.4 g / mL 1,2-propylene glycol and 90-99% basal culture medium or sodium chloride for injection by volume. By using the immune cell cryopreservation solution for immune cell cryopreservation, the survival rate of recovery cells can reach 93% or above, the biological property of the cells is not changed, and the biological activity of the immune cells is ensured; in addition, due to the fact that no animal serum is contained in the immune cell cryopreservation solution, foreign protein cannot be introduced, the possibility of animal pathogeny contamination is reduced, and the problems that immune cells cannot be stored and transported for a long time are effectively solved.

Description

technical field [0001] The invention relates to the field of cryopreservation of cells, in particular to the field of cryopreservation of immune cells, in particular to an immune cell cryopreservation solution and its application. Background technique [0002] As an effective method to preserve cells, cell freezing technology has been widely used in the field of biology. Cryopreservation of donor immune cells can maintain their vitality and function, which is of great significance for both clinical and basic research, especially when immune cells are used for retrospective research. Cryopreservation of immune cells can not only solve the problem of long induction time of existing immune cells, multiple inductions and multiple blood collections from patients, but also preserve immune cells in the best health state or the strongest combat effectiveness period for use in tumors treatment and anti-aging health care. [0003] Cell damage is normally caused when liquids freeze, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221
Inventor 刘军唐欢
Owner 居李生物科技(北京)有限公司
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