Megakaryotic progenitor cell cryopreservation medium and its application
A technology of megakaryotic progenitor cells and cryopreservation solution, which is applied in the field of bioengineering and can solve the problems of lack of cryopreservation research on megakaryotic progenitor cell types
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Embodiment 1
[0034] The inventor adopts 7 kinds of cryopreservation solution containing only DMSO and culture medium, commercially available 07930 cryopreservation solution, plasma-free cryopreservation solution containing HSA, and plasma-containing cryopreservation solution of the present invention, respectively. The megakaryotic progenitor cells induced and expanded in vitro were cryopreserved, and the cryopreservation effect was compared to observe the effects of umbilical cord plasma and poloxamer on the cryopreservation of megakaryotic progenitor cells, as follows:
[0035] 1. Experimental method
[0036](1) Mononuclear cells from umbilical cord blood were isolated, megakaryotic progenitor cells were induced and expanded for 10 days in vitro, and expanded megakaryotic progenitor cells were obtained.
[0037] (2) The megakaryotic progenitor cells obtained on the 10th day of above-mentioned in vitro induction and expansion were cryopreserved using seven kinds of cryopreservation solutio...
Embodiment 2
[0045] The megakaryotic progenitor cells were cryopreserved according to the method of Example 1, except that the frozen cells were megakaryotic progenitor cells induced in vitro for 14 days, and the components of some cryopreservation solutions were different. The specific components of the cryopreservation solutions are shown in Table 2.
[0046] Table 2 Effects of different cryopreservation solutions on the cryopreservation effect of megakaryotic progenitor cells induced in vitro for 14 days (n=3)
[0047]
[0048] The results showed that plasma can effectively improve the survival rate of cryopreservation solution, and the number of recovered cells is large, and within a certain range, with the increase of plasma concentration, the survival rate of cells and the number of recovered cells also increased. Moreover, P188 and P407 can further increase the survival rate of cells and the number of recovered cells to a certain extent.
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