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4-Terpineol type Melaleuca alternifolia tissue culture subculture propagation method and its culture medium

A technology of subculture proliferation and culture medium, which is applied in the field of 4-terpineol-type Melaleuca alternifolia tissue culture subculture proliferation method and its culture medium, and can solve the problem of large genetic differentiation of seedlings and the seeds of Melaleuca alternifolia. Small, the germination rate is only 10%, etc.

Active Publication Date: 2018-06-29
GUANGXI FORESTRY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The seeds of Melaleuca alternifolia are small, the germination rate is only 10%, and the yield is low, which is difficult to meet the needs of production
At the same time, the genetic differentiation among individual seedlings is large, and the biomass, essential oil content and components of a single plant are uneven, which restricts the improvement of essential oil production.

Method used

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  • 4-Terpineol type Melaleuca alternifolia tissue culture subculture propagation method and its culture medium
  • 4-Terpineol type Melaleuca alternifolia tissue culture subculture propagation method and its culture medium
  • 4-Terpineol type Melaleuca alternifolia tissue culture subculture propagation method and its culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Selection of excellent individual plants

[0024] Select excellent individual plants with high biomass and high oil content in the Melaleuca alternifolia forest, whose essential oil index reaches the international standard IS04730-2004 for "tea tree oil", its 4-terpineol content> 35%, 1,8-eucalyptus Leaf element content <5%.

[0025] Acquisition of initial buds

[0026] 2.1. Collection of explants

[0027] On the basis of the selection of excellent individual plants, from November to December of the previous year, the selected 4-terpineol type Melaleuca alternifolia excellent individual plants were felled or circumcised to promote germination (tree cutting 1 / 2 of the circumference), cut to the phloem. When the sprouts grow to 30-40cm, they are collected and inoculated in the afternoon on a sunny day.

[0028] 2.2. Disinfection of explants

[0029] After removing the leaves from the branches taken back, clean the soil and other pollutants attached to the surface o...

Embodiment 2

[0038] Selection of excellent individual plants

[0039] Select excellent individual plants with high biomass and high oil content in the Melaleuca alternifolia forest, whose essential oil index reaches the international standard IS04730-2004 for "tea tree oil", its 4-terpineol content> 35%, 1,8-eucalyptus Leaf element content <5%.

[0040] Acquisition of initial buds

[0041] 2.1. Collection of explants

[0042] On the basis of the selection of excellent individual plants, from November to December of the previous year, the selected 4-terpineol type Melaleuca alternifolia excellent individual plants were felled or circumcised to promote germination (tree cutting 2 / 3 of the circumference), cut to the phloem. When the sprouts grow to 30-40cm, they are collected and inoculated in the afternoon on a sunny day.

[0043] 2.2. Disinfection of explants

[0044]After removing the blades from the branches taken back, clean the dirt and other pollutants attached to the surface of...

Embodiment 3

[0053] Selection of excellent individual plants

[0054] Select excellent individual plants with high biomass and high oil content in the Melaleuca alternifolia forest, whose essential oil index reaches the international standard IS04730-2004 for "tea tree oil", its 4-terpineol content> 35%, 1,8-eucalyptus Leaf element content <5%.

[0055] Acquisition of initial buds

[0056] 2.1. Collection of explants

[0057] On the basis of the selection of excellent individual plants, from November to December of the previous year, the selected 4-terpineol type Melaleuca alternifolia excellent individual plants were cut down or circumcised to promote germination (circumcision) for a full week), cutting down to the phloem. When the sprouts grow to 30-40cm, they are collected and inoculated in the afternoon on a sunny day.

[0058] 2.2. Disinfection of explants

[0059] After removing the blades from the branches taken back, clean the dirt and other pollutants attached to the surfac...

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Abstract

The invention discloses a 4-terpineol-type Melaleuca alternifolia tissue culture subculture propagation method. In the method, an excellent individual plant of the 4-terpineol-type Melaleuca alternifolia is selected for pile cutting or circumcision to promote germination. Germination strips were collected and cultured in the specially designed primary induction medium and secondary proliferation medium. Experiment shows, application of the present invention can improve bud organ reproduction coefficient, shortens bud organ subculture propagation cycle, increases effective bud number, thereby reduces the production cost in 4 terpineol type Melaleuca alternifolia tissue culture industrialized seedling cultivation; The explants are collected from 4‑terpineol-type Melaleuca alternifolia excellent individual plants, and its tissue culture seedlings maintain the excellent genetic traits of the female parent, so that the Melaleuca alternifolia seedlings can be improved and cloned, and the unit can be increased The biomass and oil production of the area can be improved, and the economic and ecological benefits of the Melaleuca forest stand can be improved.

Description

technical field [0001] The invention belongs to the technical field of tissue culture and seedling cultivation of Melaleuca alternifolia, in particular to a 4-terpineol type Melaleuca alternifolia tissue culture subculture propagation method and a culture medium thereof. Background technique [0002] Melaleuca alternifolia Linn is a plant of the Myrtaceae family Melateuca, commonly known as tea tree, evergreen shrub to small tree, native to Queensland and northern New South Wales in eastern Australia . Because the branches and leaves of Melaleuca alternifolia contain volatile components such as α-terpinene, eucalyptol, γ-terpinene, 4-terpineol, etc., it has been widely planted as a spice plant in Australia for a long time. The price of different biochemical types of Melaleuca alternifolia oil varies greatly, and the uses are also different; 4-terpineol type Melaleuca alternifolia oil, which is often called tea tree oil, has the highest value; eucalyptol type Melaleuca alter...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
CPCA01H4/00A01H4/001
Inventor 刘海龙吴丽君覃子海陈博雯肖玉菲张烨姚瑞玲张晓宁
Owner GUANGXI FORESTRY RES INST
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