Nucleic acid detection method and detection tube

Abstract

The invention discloses a nucleic acid detection method and a detection tube. A plurality of separation layers are arranged in the detection tube to isolate the lysate, cleaning liquid and reaction liquid in the detection tube. Different stages are melted separately to control the timing of each detection stage, and a magnetized mixing device controlled by an external magnetic body is used in the lysate to mix the sample added to the detection tube and realize the nucleic acid extraction and purification of the sample. Then use an external magnetic body to drive the nano-magnetic beads in the detection tube through the cleaning solution and then enter the reaction solution. The nucleic acid carried by the nano-magnetic beads is purified in the cleaning solution and then eluted in the reaction solution. The nucleic acid is amplified in the reaction solution. Finally, the external device realizes the detection of sample nucleic acid through optical detection, thereby realizing multiple steps of nucleic acid extraction and purification, cleaning, elution and amplification reaction in the same detection tube.

Description

technical field [0001] The invention relates to a nucleic acid detection method and a detection tube, in particular to a nucleic acid detection method and a detection tube mainly used in nucleic acid extraction and amplification technologies. Background technique [0002] Laboratory testing is an important means of screening and diagnosing bacterial or viral infections, mainly including microscopy, culture, immunological testing and nucleic acid testing. Microscopic examination is low in cost, simple, and convenient, but the positive rate is low and it is easy to miss; the results of culture method are highly reliable, but the cycle is long and the cost is high; immunological detection is easy to operate, but there is a window period, so it is easy to miss The nucleic acid detection cycle is short, and the sensitivity and specificity are comparable to those of the culture method, but there are the following problems: ①The operation process of sample preparation (nucleic acid...

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Problems solved by technology

Microscopic examination is low in cost, simple, and convenient, but the positive rate is low and it is easy to miss; the results of culture method are highly reliable, but the cycle is long and the cost is high; immunological detection is easy to operate, but there is a window period, so it is easy to miss The nucleic acid detection cycle is short, and the sensitivity and specificity are comparable to those of the culture method, but there are the following problems: ①The operation process of sample preparation (nucleic acid extraction), nucleic acid amplification and amplified product detection is carried out separately, which requires the operation of professional operators There are many and cumbersome steps, which increases operational errors; ②Excessive manual operations can easily cause cross-contamination of samples and false positive test results; ③Most nucleic acid amplification reagents need cold chain transportation and cryopreservation, and the cost is relatively high. high

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