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PIK3CA gene mutation detection kit

A technology for detection kits and detection reagents, applied in the field of gene mutation detection, to achieve the effects of reducing time loss and economic loss, improving treatment effect, avoiding the burden of medical expenses for patients and the waste of social medical resources

Inactive Publication Date: 2016-01-27
BEIJING ACCB BIOTECH
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Problems solved by technology

[0007] Carrying PIK3CA mutation may lead to resistance to gefitinib in lung cancer patients, study finds [8] , leading to resistance to trastuzumab in breast cancer patients [9-11] , leading to resistance to cetuximab and panitumumab in colorectal cancer patients [12-14]

Method used

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Embodiment Construction

[0024] 1. Experimental method

[0025] Using real-time fluorescent PCR technology. The gene mutation was detected by ARMS (amplification refractory mutation system) method. That is, the method of identifying the mutation at the 3' end of the primer is combined with the hydrolysis and luminescence of the TaqMan probe to detect the gene mutation.

[0026]The kit contains internal reference gene detection and internal control gene detection. The internal reference gene (Internal Reference, IR) is a housekeeping gene different from the gene PIK3CA to be tested. By detecting the amplification of the internal reference gene (FAM channel), it can be analyzed whether the DNA to be tested can be amplified normally, so as to rule out the reasons for the failure of PCR detection such as DNA purity, poor concentration, or containing PCR inhibitors. This kit is also equipped with an internal control gene (Internal Control, IC) detection system in the detection system of each mutation ty...

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Abstract

The present invention relates to a PIK3CA gene mutation detection kit, which can be used for detection of PIK3CA mutations associated with cancer.

Description

technical field [0001] The invention relates to gene mutation detection. Specifically, the present invention relates to a PIK3CA gene mutation detection kit, which can be used to detect cancer-related PIK3CA gene mutations. Background technique [0002] The incidence of malignant tumors in China has been increasing year by year in recent years, with more than 1.7 million new patients each year. Lung cancer is one of the most common malignant tumors, and its mortality rate ranks first in all kinds of malignant tumors. Among them, non-small cell lung cancer (NSCLC) has the highest incidence rate, accounting for about 80% of all lung cancers. Colorectal cancer is a malignant tumor of the digestive tract, and its incidence ranks fourth among common tumors. Breast cancer is a common malignant tumor in women, and there are about 180,000 new cases of breast cancer in my country every year. [0003] The U.S. Food and Drug Administration (FDA) approved the TKI drugs Gefitinib (Gef...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6886C12Q2600/156C12Q2600/16C12Q2600/166C12Q1/68
Inventor 莫敏俐李晖丁凤陈钊李隽
Owner BEIJING ACCB BIOTECH
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