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Common polypeptide in animal glue and application thereof in detection

A technology of animal glue and polypeptide, which is applied to peptides, measuring devices, material separation, etc., can solve the problems of using horsehide, cowhide, pigskin, sheepskin, tortoise shell, antlers, and large deviations in the reported literature

Active Publication Date: 2016-01-27
SHAN DONG DONG E E JIAO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the reported literature, animal glues made of horse hide, cowhide, pigskin, sheepskin, tortoise shell, deer antler, etc. can be identified and distinguished, but it is difficult to find cases of falsification by mixing other animals as raw materials
Animal glue is generally concentrated by long-term high-temperature cooking, and the protein and polypeptide in the glue are hydrolyzed to varying degrees. For animal glue produced by different processes, mass spectrometry detection methods produce large deviations in the detection of different animal component contents

Method used

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  • Common polypeptide in animal glue and application thereof in detection
  • Common polypeptide in animal glue and application thereof in detection
  • Common polypeptide in animal glue and application thereof in detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The detection of consensus polypeptide in the animal glue of embodiment 1

[0025] 1. Materials and reagents

[0026] Materials: Pure glue includes donkey-hide gelatin, horsehide glue, yellow gelatin, fresh donkey-hide gelatin, tortoise shell glue, turtle shell glue, antler glue, sheepskin glue, donkey hide, horse hide, cowhide, pig skin, tortoise shell, turtle shell, antler respectively 1. Sheepskin is obtained by decocting, extracting, concentrating and drying.

[0027] Reagents: ammonium bicarbonate (analytical grade), trypsin (serial grade, purchased from China National Institutes for Food and Drug Control).

[0028] 2. Detection method

[0029] (1) Preparation of gelatin sample enzymatic hydrolysis solution

[0030] Get 0.10g of donkey-hide gelatin, the sample to be tested, in a 50ml measuring bottle, add an appropriate amount of 1% (w / w) NH 4 HCO 3 Solution, sonicate to dissolve the sample completely, with 1% (w / w) NH 4 HCO 3 Dilute the solution (pH8.0) to ...

Embodiment 2

[0035] Example 2 Application of Consensus Polypeptides in Animal Glue Authenticity Identification

[0036] 1. Materials and reagents

[0037] Materials: Fish gelatin (purchased from Guangdong Mingyang Gelatin Co., Ltd.), donkey-hide gelatin and yellow gelatin are obtained by decocting, concentrating, extracting and drying from donkey hide and cowhide respectively.

[0038] Mixed gum samples were prepared by adding 30% (w / w) fish gelatin, 60% (w / w) fish gelatin, 30% (w / w) yellow gelatin and 60% (w / w) yellow gelatin from donkey-hide gelatin. Ejiao with %(w / w) fish gelatin, donkey-hide gelatin with 60% (w / w) fish gelatin, donkey-hide gelatin with 30% (w / w) yellow gelatin, donkey-hide gelatin with 60% (w / w) yellow gelatin Test samples.

[0039] Reagents: ammonium bicarbonate (analytical grade), trypsin (serial grade, purchased from China National Institutes for Food and Drug Control).

[0040] 2. Detection method

[0041] (1) Preparation of gelatin sample enzymatic hydrolysis so...

Embodiment 3

[0056] Example 3 Application of Consensus Polypeptides in Detection of Animal Component Content in Animal Glue

[0057] 1. Materials and reagents

[0058] Materials: Homemade donkey-hide gelatin samples with different decoction conditions were all obtained by decocting, concentrating and drying with donkey skin (decoction conditions are different, others are the same), among them, 1# self-made donkey-hide gelatin was decocted with tap water, and the decoction temperature was 110℃ ; 2# self-made donkey-hide gelatin is decocted with 1% (w / w) NaOH aqueous solution, and the decoction temperature is 110°C; 3# self-made donkey-hide gelatin is decocted with tap water, and the decoction temperature is 130°C; 4# self-made donkey-hide gelatin is decocted with 1% NaOH The aqueous solution is decocted, and the decoction temperature is 130°C.

[0059] Reagents: ammonium bicarbonate (analytical pure), trypsin (serial pure, purchased from China National Institutes for Food and Drug Control)...

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Abstract

The invention discloses a common polypeptide in animal glue and application thereof in detection. The common polypeptide provided by the invention has a sequence of Gly-Ser-Ala-Gly-Pro-Hyp-Gly-Ala-Thr-Gly-Phe-Hyp-Gly-Ala-Ala-Gly-Arg. The polypeptide is common in the collagen sequence of donkeys, horses, cattle, pigs, sheep, turtles, tortoises, deer and other animals, and suffers a basically identical degree of production process destruction to that of collagen polypeptide components. Therefore, use of the common polypeptide as a reference substance or internal standard substance in mass spectrometry detection can offset different influences of the destructive degrees of different production processes, the content determination can be more accurate, and also whether other animal ingredients besides donkeys, horses, cattle, pigs, sheep, turtles, tortoises, deer are doped can be found timely.

Description

technical field [0001] The invention relates to a common polypeptide in animal glue and its application in detection, belonging to the field of traditional Chinese medicine detection. Background technique [0002] Animal glue is a kind of glue made from the skin and bone of animals. Common ones include donkey-hide gelatin, tortoise shell glue, deer antler glue, and turtle shell glue. Various glues have their own characteristics in terms of pharmacology and clinical application. All kinds of glue must be boiled with specified animals, such as donkey-hide gelatin boiled from donkey skin, yellow gelatin boiled from cowhide, tortoise shell glue boiled from tortoise shell, etc. Otherwise, it will be regarded as fake and inferior. product. Because the prices of various glues vary greatly and their appearances are very similar, criminals who counterfeit and manufacture counterfeit products have appeared in the market. Taking donkey-hide gelatin as an example, in the production of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08G01N30/02
Inventor 周祥山郝向慧郭尚伟段小波田守生嵇传良徐云鹏王静史兆松张云霞
Owner SHAN DONG DONG E E JIAO
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