Application of Bic Angelica Lactone
A technology of Baccarina lactone and notopterygium, applied in the field of medicine, to achieve the effect of protecting kidney damaged cells and wide application prospects
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Embodiment 1
[0024] Embodiment 1: Extraction and separation of Bic Angelica lactone
[0025] Grind the rhizome of notopterygium into a coarse powder, add 6-10 times of 70% ethanol solution according to volume / mass to extract twice, each time for 1.5 hours, combine the filtrate, concentrate until it has no alcohol smell, then add water to 1 times the weight of the medicinal material; concentrate Concentrated hydrochloric acid was added to the solution to adjust the pH to 2, heated to reflux for 0.5 hours, concentrated and evaporated to dryness, and 40% ethanol solution of 1 times the weight of the medicinal material was added to completely dissolve it. After the hydrolyzate is absorbed by a D-101 macroporous resin column (diameter-to-height ratio: 1:8) twice the weight of the medicinal material, it is eluted with water for 4 column volumes, 40% ethanol for 3 column volumes, and 60% ethanol for elution. 6 column volumes. The 60% ethanol eluents were combined, the solvent was recovered, and ...
Embodiment 2
[0030] Example 2: Protective effect of baccaratone on cisplatin-induced HK-2 cell kidney injury model
[0031] 1. Materials and methods
[0032] 1.1 Cells Human renal tubular epithelial cells HK-2 were purchased from China Center for Type Culture Collection.
[0033] 1.2 Main reagents and instruments
[0034] Cisplatin was purchased from sigma company; Dulbecco's minimum essential medium (DMEM) / F12 culture medium, fetal bovine serum (FBS) and trypsin were purchased from Gibco, USA; MTT was purchased from Biyuntian company.
[0035] 1.3 Culture of HK-2 cells
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