Aristolochone b and its preparation method and application
A kind of technology of aristolochone and pine, applied in the field of plant extract and preparation thereof
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Embodiment 1
[0033] Preparation of active ingredient aristolochone B (extraction and separation process):
[0034] The decoction pieces of Gansong medicinal materials were purchased from Anhui Jiren Pharmaceutical Co., Ltd. (batch number: 110709, specification: 1kg / bag, place of origin: Sichuan), about 20kg, and 20kg of Gansong rhizome was extracted with 70% ethanol for 3 times, each time for 48 hours. Combine the extracts, concentrate under reduced pressure to obtain 3 kg of crude extract extract; then use 70% (v / v) ethanol to heat extract the above-mentioned medicinal residues for 3 times, each time for 2 hours, combine the extracts, concentrate under reduced pressure to obtain crude extract Extraction extract 400g; Merge two crude extracts to obtain total extract 3.4kg; Gained crude extract total extract, after water dispersion, extract with equal volume of sherwood oil, ethyl acetate, n-butanol successively, obtain Petroleum ether part 320g, ethyl acetate part 1kg, n-butanol part 600g,...
Embodiment 2
[0038] The influence of aristolochone B of the present invention on 5-hydroxytryptamine transporter (SERT)
[0039] Using stably transfected hSERT-HEK293 cell line, 4-(4-(dimethylamino)phenyl)-1-methylpyridinium (APP + ) is a fluorescent substrate, and the effect of compound aristolochone B on SERT activity was detected on a high-content system.
[0040] 1) Experimental instruments and reagents
[0041] laboratory apparatus:
[0042] High-content Operetta system and Columbus data management and analysis system (PerkinElmer), ultra-clean bench, pipette gun (1000μL, 200μL, 20μL, 10μL, 2.5μL, Eppendorf, USA)
[0043] Reagents and materials:
[0044] Human embryonic kidney cell line HEK293 (Cell Bank of Type Culture Collection Committee, Chinese Academy of Sciences), hSERT pcDNA3 plasmid (Addgene, plasmid 15483), MEM medium (Gibco), APP+ (Sigma), Hoechst 33342 (CellSignaling Technology), 96-well plate (Costar 3605)
[0045] 2) Experimental operation process
[0046] First es...
Embodiment 3
[0065]
[0066] Preparation method: uniformly mix aristolochone B, lactose and starch according to the above ratio, pass through a 200-mesh sieve, moisten evenly with water, dry the wetted mixture and sieve, add magnesium stearate, and then The mixture is compressed into tablets, each tablet weighs 250 mg, and the active ingredient content is 10 mg.
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