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A promoter regulating gene expression in non-secreting glandular trichomes and its application

A non-secretory glandular hair and gene regulation technology, applied in the field of promoters, can solve problems such as excessive consumption of cellular substances and energy, regulation of target genes in time and space, and normal growth burden of plants.

Active Publication Date: 2017-11-10
SHANGHAI JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the current genetic engineering research of Artemisia annua, constitutive promoters are often used, such as the cauliflower mosaic virus 35S promoter (CaMV35S), which can drive the expression of foreign genes in all tissues and organs, and will cause excessive consumption. The substance and energy in the cell, and the expression of the target gene cannot be regulated in time and space, which is likely to cause a certain burden and harm to the normal growth of plants

Method used

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  • A promoter regulating gene expression in non-secreting glandular trichomes and its application
  • A promoter regulating gene expression in non-secreting glandular trichomes and its application
  • A promoter regulating gene expression in non-secreting glandular trichomes and its application

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Embodiment

[0038] This embodiment relates to the acquisition of the AaADH2 gene promoter, which specifically includes the following steps:

[0039] Step 1. Cultivate aseptic seedlings of Artemisia annua

[0040] Artemisia annua seeds were soaked in ethanol with a volume fraction of 75% for 1 min, then soaked in 20% (w / v) NaClO for 20 min, rinsed with sterile water for 3 to 4 times, blotted the surface moisture with sterile absorbent paper, and inoculated Cultivate on a hormone-free MS solid medium at 25°C under 16h / 8h (light / dark) light, and after 14 days, you can obtain an Artemisia annua sterile vaccine;

[0041] Step 2: Cloning of the promoter sequence in the genomic DNA

[0042] Step 1. Cultivate aseptic seedlings of Artemisia annua

[0043] Artemisia annua seeds were soaked in ethanol with a volume fraction of 75% for 1 min, then soaked in 20% (w / v) NaClO for 20 min, rinsed with sterile water for 3 to 4 times, blotted the surface moisture with sterile absorbent paper, and inoculat...

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Abstract

The invention discloses a gene promoter in the field of plant biotechnology. The nucleotide sequence of the promoter is shown in SEQ ID NO: 1, which can regulate the specific expression of target genes in plant non-secretory glandular trichomes. At the same time, the present invention also relates to the use of the aforementioned gene promoter in genetic engineering breeding for expression and production of metabolites by plant glandular hair tissue. The growth and development of the plant will not be harmed when the plant glandular hair system is genetically manipulated by using the promoter specifically expressed by the plant glandular hair. Therefore, the present invention is of great significance to genetic engineering breeding utilizing plant glandular hair tissue to express and produce metabolites.

Description

technical field [0001] The present invention relates to a promoter, in particular to a terpene synthase gene promoter (proADH2) obtained by means of molecular biology and specifically expressed in plant non-secretory glandular hairs and its application in transgenic plants . Background technique [0002] Artemisia annua L. is an annual herb of the genus Artemisia in the Asteraceae family. The plant has a strong volatile aroma, and its plant contains many secondary metabolites: artemisinin, volatile oil, α-pinene, camphor, artemisia annua Ketones, etc., in addition to flavonoids. Artemisinin (Artemisinin) is a sesquiterpene lactone compound containing a peroxo bridge structure isolated from its aerial part, as an antimalarial drug combination therapy (Artemisinin-based combination therapies, ACTs) are the main active ingredients. At present, the main source of artemisinin is extracted from the aerial parts of Artemisia annua, however, the content of artemisinin in Artemisi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113C12N15/82C12Q1/68A01H5/00
Inventor 唐克轩石璞付雪晴沈乾孙小芬陈明慧马亚男郝小龙
Owner SHANGHAI JIAOTONG UNIV
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