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Method for Rapid Propagation and Raising Seedlings by Utilizing Aesculus Aesculus Rhizome

A technique of aesculus aesculus and rhizomes, which is applied to the propagation field of rapidly inducing seedlings by dividing the rhizomes of aesculus aesculus, can solve the problems of low cultivation environment, short cultivation time and high application value, and achieves high transplanting survival rate. , increase the reproduction coefficient, the effect of high application value

Active Publication Date: 2017-05-03
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0024] Due to the low reproduction coefficient of Aesculus aesculus through bud heads, and the operation steps are many, the process is complicated and requires aseptic operation environment and related operating technicians through tissue culture technology, resulting in large investment and low output through tissue culture. The problem is not high, so the purpose of the present invention is to provide a method for rapid propagation of Aesculus aesculata with low cultivation environment, low investment and high reproduction coefficient, which is suitable for industrial production, with good formula effect, high germination rate and short cultivation time , strong plant growth, strong maneuverability, high application value, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Choose the fresh rhizomes of Aesculus aesculata excavated on January 20, and wash them with 7% carbendazim solution. Cut off the original growth point, that is, the terminal bud, destroy the original growth point, and cut off the rhizome without long fibrous roots, and the rhizome without bud head is used for medicinal purposes. Cut 10 kg of each Aescin flower bud head vertically into 4 to 8 small pieces, mix with 15 kg of 5 mesh granular activated carbon after sterilization, spray the bud growth promoting solution once every three days, and the bud growth promoting solution contains 3g The aqueous solution of 6-BA, 0.2g / L NAA, 4% carbendazim per 100kg of Aesculus chinensis rhizome is sprayed with 10kg / time of germination-promoting solution, and the relative humidity is maintained at 80%, and the light intensity is 400~600LX. Illuminated for 10 hours a day, the temperature was 18°C, and sprouts grew in 28 days. After the buds grow out, treat them three times with the r...

Embodiment 2

[0048] Choose the fresh rhizome of Aesculus chinensis excavated on February 5, and wash it with 8% carbendazim solution. Cut off the original growth point, that is, the terminal bud, destroy the original growth point, and cut off the rhizome without long fibrous roots. 10 kg of each Aescin flower bud head is vertically cut into 4 to 8 small pieces, and then mixed with 16 kg of 4 mesh granular activated carbon after sterilization, spraying 1.2 kg of a bud growth-promoting solution every three days, and the bud growth-promoting solution is An aqueous solution containing 4g / L 6-BA, 0.4g / L NAA, and 5% carbendazim is maintained at a relative humidity of 80%, with a light intensity of 300-400LX, 10 hours of light per day, and a temperature of 19°C, and sprouts grow in 25 days. After the bud grows, it is processed three times with the solution for cultivating roots, and the three-time treatment interval is 4 days. The solution for cultivating roots is sprayed with 1.8kg / time of every...

Embodiment 3

[0050] Choose the fresh rhizomes of Aesculus aesculi that was excavated on January 20, and wash them with 9% carbendazim solution. Cut off the original growth point, that is, the terminal bud, destroy the original growth point, and cut off the rhizome without long fibrous roots. Cut 10 kg of each Aescin flower bud head vertically into 4 to 8 small pieces, mix them with 20 kg of sterilized 3-mesh granular activated carbon, spray the germ growth-promoting solution once every three days, and the germ-promoting solution contains 2.5 The aqueous solution of g / L 6-BA, 0.3g / L NAA, 4% carbendazim, every 100kg Aesculus chinensis rhizome sprays 13kg / time of germination-promoting solution, maintains relative humidity at 85%, light intensity 400~600LX , light 10h a day, temperature at 16 ℃, 32 days to grow buds. After the buds grow out, treat them three times with the root-raising solution. The root-raising solution is an aqueous solution containing 1g / L NAA and 2% carbendazim. kg / time,...

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Abstract

The invention provides a rapid propagation and seedling culture method using rhizomes of paris polyphylla. According to the method, rapid propagation is realized by the aid of the rhizomes of paris polyphylla, large quantity of complete plants are obtained after bud inducing and root inducing processing, and large-scale industrial seedling culture of paris polyphylla is realized. The method has the advantages that an inhibiting action between potential buds on single rhizome of paris polyphylla can be avoided completely, the propagation coefficient is increased to the greatest extent, the genetic stability of the paris polyphylla breed is guaranteed sufficiently, and high probability of variation of seeds and tissue culture seedlings is eliminated. Compared with other propagation methods using the rhizomes, completely independent plants can be formed and seedlings with multiple buds can be further formed with the method.

Description

technical field [0001] The invention relates to the field of medicinal plant seedling breeding engineering, in particular to a propagation method for quickly inducing seedlings by dividing the rhizome of Aesculus aesculi. Background technique [0002] Aescin is a perennial herbaceous plant, also known as unipedal lotus, one arrow, grass river car and so on. Wild Aescin is distributed in Hunan, Yunnan, Sichuan, Guizhou and other provinces. The main production areas are Xiangxi in Hunan, Central Yunnan, Western Yunnan and Northwestern Yunnan. It grows at an altitude of 1400-3100m, with an annual average temperature of 10 ~ 13 ℃, annual rainfall 850 ~ 1200mm in cool mountainous areas, mostly in evergreen broad-leaved forests, coniferous and broad-leaved mixed forests, damp places on hillsides and shrublands. Aescin germinates and grows after 5 to 6 years from the seed, and then enters the reproductive growth period. Due to its slow growth, the resources of wild Aesculus aescu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 覃静萍唐忠海
Owner HUNAN AGRICULTURAL UNIV
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