Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Extraction method of duck intestinal tract antimicrobial peptide

An extraction method and antimicrobial peptide technology, which is applied in the field of duck intestinal antimicrobial peptide extraction, can solve the problems of separation and purification difficulties, complex antimicrobial peptide methods, etc., and achieve the effect of high separation and extraction rate, low cost and simple operation

Inactive Publication Date: 2015-10-14
CHONGQING DOUHAO BIOTECH
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the above-mentioned problems existing in the prior art field, the object of the present invention is to provide a method for extracting duck intestinal antimicrobial peptides, which solves the problems that the current methods for extracting antimicrobial peptides are relatively complicated and difficult to separate and purify

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0022] A kind of extraction method of duck intestinal antimicrobial peptide of the present embodiment is characterized in that it comprises the following specific steps:

[0023] 1) Preparation of crude product

[0024] (1) Get the duck intestinal mucosa and wash it with sterilized saline;

[0025] (2) Add 5% acetic acid at a ratio of 1:1 (W / V);

[0026] (3) Use a tissue masher to mash 3 times, each time for 2 minutes;

[0027] (4) 100°C water bath for 10 minutes, then rapidly cooled, centrifuged at 8000 rpm for 30 minutes to discard the precipitate, and collect the supernatant;

[0028] (5) Put the supernatant on ice and ultrasonically break it 10 times, 3s each time; stir and extract at 4°C for 24 hours, centrifuge at 8000r / min for 30min, and take the supernatant;

[0029] (6) Mix the precipitate with an equal volume of 5% acetic acid, stir and extract again, and centrifuge to get the supernatant;

[0030] (7) Combine the two supernatants, adjust the pH value to 7.0, and...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an extraction method of a duck intestinal tract antimicrobial peptide, which comprises the following steps: (1) preparation of antimicrobial peptide crude product; and (2) purification of the antimicrobial peptide crude product. The method is simple to operate, has the characteristics of high separation and extraction rate and low cost, and is suitable for separating and purifying abundant antimicrobial peptides from duck intestines.

Description

technical field [0001] The invention relates to an extraction method of antibacterial peptides, in particular to an extraction method of duck intestinal antibacterial peptides. Background technique [0002] Due to the long-term widespread use of traditional antibiotics to treat infections, many drug-resistant strains have emerged. Therefore, the development of a new antibacterial drug has become the main target of many medical scientists and microbiologists. Antimicrobial peptides with a new antibacterial mechanism have obvious advantages in this respect. [0003] The intestinal tract not only has the function of digestion and absorption, but also has a very important endocrine function. There are many antimicrobial peptides with physiological activity in the intestinal tract. Studies have found that antimicrobial peptides have special significance in the natural immunity of the intestinal mucosa of the body, and are direct effector molecules of antimicrobial immunity. Ant...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K14/465C07K1/36C07K1/16C07K1/14
CPCC07K14/465
Inventor 肖继忠宁发子邓小玲
Owner CHONGQING DOUHAO BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com