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A kind of sensor preparation method and application based on screen printing carbon electrode

A technology of screen printing and carbon electrodes, which is applied in the field of biosensors, can solve the problems of low residue and quantitative detection, inaccurate detection results, and the influence of spectrophotometry, etc., and can be mass-produced at cost, with high stability and sensitivity , design flexible effects

Active Publication Date: 2018-05-22
SHANDONG EMBER INSTR
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The principle of spectrophotometry is based on the change of absorbance for detection, but a large number of pigments in vegetables and fruits will have a great impact on spectrophotometry, resulting in inaccurate detection results
Moreover, the above method has disadvantages such as low recovery rate, high proportion of false detection and missed detection, poor repeatability, and difficulty in meeting the requirements of low residue and quantitative detection.

Method used

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  • A kind of sensor preparation method and application based on screen printing carbon electrode
  • A kind of sensor preparation method and application based on screen printing carbon electrode
  • A kind of sensor preparation method and application based on screen printing carbon electrode

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Embodiment 1: A kind of sensor preparation method based on screen printing carbon electrode

[0058] (1) Cleaning of the screen-printed carbon electrode before the preparation of the amperometric acetylcholinesterase sensor, place the screen-printed carbon electrode in 1 mM NaOH solution for 5 min, rinse with ultrapure water, and N 2 blow dry. Afterwards, the electrode was placed in 1mM HCl solution for 5min sonication, rinsed with ultrapure water, N 2 blow dry. Finally, rinse the electrode with absolute ethanol, N 2 blow dry;

[0059] (2) Activation of the electrode before the preparation of the amperometric acetylcholinesterase sensor, scanning the current-time curve for 300 s in pH 5.0 phosphate buffer solution, and then scanning the cyclic voltammetry curve until the performance is stable;

[0060] (3) The preparation of dicyclohexyl phthalate / multi-walled carbon nanotubes, 0.1g chitosan is joined in 50mL concentration and is 1.0% acetic acid solution, is made i...

Embodiment 2

[0062] Example 2: Using the prepared amperometric acetylcholinesterase sensor to detect vegetable samples

[0063] (1) The acetylcholinesterase sensor prepared according to Example 1 is subjected to a cyclic voltammetry test at a scan speed of 50 mV / s in a phosphate buffer solution of pH 7.5 containing 1.0 mM thioacetylcholine chloride (ATCl), The potential window is 0V ~ 1.0V;

[0064] (2) Configure chlorpyrifos standard solution. When measuring pesticides, immerse the above-mentioned acetylcholinesterase sensor in different concentrations of pesticide standard solutions for 10 minutes, and then add 15 to 20 mL of chlorpyrifos containing 1.0 mM thioacetylcholine chloride (ATCl) to the reaction pool. Phosphate buffered saline, carry out cyclic voltammetry scan, and inhibition rate I can be obtained by following formula:

[0065] I(%)=(I P,control -I P,exp ) / I P,control ×100%

[0066] where I P,control and I P,exp Respectively, the peak current of the modified electrode ...

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Abstract

The invention discloses a method for preparing a sensor based on a screen-printed carbon electrode and its application, and belongs to the technical field of biosensors. The method provided by the present invention is to prepare dicyclohexyl phthalate / multi-walled carbon nanotube nanomaterials, which are layered and drip-coated on the surface of a screen-printed carbon electrode, and then acetylcholinesterase is drip-coated on the phthalate Dicyclohexyl formate / multi-walled carbon nanotubes modified screen-printed working electrode surface for the preparation of acetylcholinesterase biosensors for the detection of organophosphorus and carbamate pesticides. The sensor prepared by the invention has the characteristics of simple process, convenient operation, low cost, high detection sensitivity, strong electrical signal and simple electrode pretreatment, the detection limit reaches 0.05 μg / L, and is suitable for detecting pesticide residues.

Description

technical field [0001] The invention relates to a preparation method and application of a sensor based on a screen-printed carbon electrode, and belongs to the technical field of biosensors. Background technique [0002] Pesticides are important means of agricultural production, and play a very important role in the prevention and control of diseases, insects, weeds, and rodents in the production of agricultural products. However, pesticide pollution and its harmful consequences are serious, and the losses caused by pesticides to environmental pollution are various, including pollution to water environment, pollution to soil, pollution to air, impact on environmental organisms and hazards to human health, etc. At present, due to the general lack of scientific knowledge of pesticide use by users and one-sided pursuit of yield, the agricultural products currently on the market have pesticide residues to varying degrees, and their harm has increasingly attracted public attenti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26
Inventor 孙霞陈栋菲徐建光郭业民王相友
Owner SHANDONG EMBER INSTR
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