Method for isolating biochemical molecules on microarray substrate

A microarray and substrate technology, applied in biochemical equipment and methods, chemical instruments and methods, microbial measurement/testing, etc., can solve problems such as economic infeasibility, inability to apply peptide or protein libraries, etc.

Active Publication Date: 2015-09-02
SEOUL NAT UNIV R&DB FOUND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is not economically feasible to apply such methods to all different sequences, and the method is only applicable to DNA libraries
That is, disadvantageously, the method cannot be applied to peptide or protein libraries

Method used

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  • Method for isolating biochemical molecules on microarray substrate
  • Method for isolating biochemical molecules on microarray substrate
  • Method for isolating biochemical molecules on microarray substrate

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Embodiment Construction

[0024] Libraries of synthetic biochemical molecules on microarrays are often processed in batches like other library types because their physical coordinates are in close proximity to each other, making them difficult to isolate individually by conventional methods. The techniques disclosed in the present invention relate to methods for selectively separating a portion of a microarray substrate from a microarray through microstructural manipulation techniques to separate and collect biochemical molecules individually. This is possible because the desired biochemical molecules present on the microarray are physically or chemically bound to the microarray substrate. Thus, isolating substrate fractions with specific spots from the microarray has the same effect as selectively isolating specific biochemical molecules from a library.

[0025] Subsequently, the selectively collected biochemical molecules are spotted with sufficient physical separation to allow for additional post-pr...

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Abstract

Provided is a method for isolating biochemical molecules on a microarray substrate, comprising the steps of: providing the microarray substrate on which clusters of different types of biochemical molecules are attached by being classified by individual spot units which are regularly arranged; acquiring the information on the location of the individual spot in which a desired cluster among the clusters of biochemical molecules is located; placing an extraction tool for applying energy so as to isolate the desired cluster based on the information on the location; and applying energy in a contact or non-contact manner using the extraction tool so as to isolate the desired cluster from the microarray substrate.

Description

technical field [0001] The present invention relates to a method for separating biochemical molecules on a microarray substrate, and a system for separating the molecules. Background technique [0002] Microarray synthesis technology is a technology for synthesizing various biochemical molecules simultaneously and in parallel. Various biochemical molecules are immobilized on the solid substrate of the synthesized microarray with suitable intervals ranging from several centimeters (cm) to several micrometers (μm). The synthesized biochemical molecule library is used in a state of being immobilized on a solid substrate or in a solution state after being removed from the substrate. For example, the synthesized biochemical molecular library is used for various genetic information analysis, such as genome sequencing, single nucleotide polymorphism (SNP) analysis, genome-wide association study (GWAS) and gene expression analysis, etc., and the biochemical The application of mole...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50C12Q1/24C12Q1/68
CPCG01N33/50B01J19/00C12Q1/24C12Q1/6806B01J19/0046B01J2219/00441B01J2219/00452B01J2219/00531B01J2219/0054B01J2219/00608B01J2219/00659B01J2219/00689B01J2219/00711B01J2219/00716B01J2219/00722B01J2219/00725C12Q1/68
Inventor 权圣勋柳兑勋崔荣宰郑有信金孝基李镐源
Owner SEOUL NAT UNIV R&DB FOUND
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