Method for multiplying vetiver grass seedlings from stem auxiliary buds

Abstract

The invention discloses a vetiver grass sterile bud culture medium, a vetiver grass sterile bud multiplication culture medium and a method for multiplying vetiver grass seedlings from stem auxiliary buds, and belongs to the technical field of plant planting methods. According to the method, an explant selection part, an explant sterilization measure and a sterile bud culture medium formula are improved, and therefore, the contamination rate is reduced, and the occurrence rate of the sterile auxiliary buds is increased; the occurrence rate is increased to 85% from 72.9% of the prior art; the buds are robust; the generation time of the auxiliary buds is shortened to minimum 15 days from the minimum 20 days of the prior art; the multiplication culture medium formula is improved so that the contamination rate can be reduced, and the bud multiplication rate can be increased to 1: 45 from 1: 11.7 of the prior art; the final-period transplanting management is added to increase the survival rate of the seedlings, and the survival rate can be 98.7%.

Description

technical field [0001] The invention belongs to the technical field of plant planting methods and provides a method for propagating vetiver seedlings by using axillary buds of stem nodes. Background technique [0002] Vetiver (Vetiveria zizanioides L.), also known as pedima and vetiver, is a perennial herbaceous plant of the Gramineae family. It originated in India and other countries, and is now mainly distributed in Southeast Asia, India, Africa and other (sub)tropical regions. In my country, it is mainly distributed in Guangdong, Yunnan and other places. Vetiver is extremely difficult to bear fruit or does not bear fruit. It mainly reproduces by tillers. That is, every spring, the roots of vetiver left in the field in the previous year will germinate new tillers. Generally, each pocket of vetiver roots can grow 40% in spring. Tillers around the plant. Theoretically speaking, each tiller can grow into an independent stem with 6-10 nodes. In fact, only the larger tillers (...

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Problems solved by technology

[0009] 2. The use of vetiver explants to induce callus differentiation to increase the reproduction coefficient has problems such as high technical difficulty, difficulty in inducing callus cells, low germination rate, and difficulty in controlling the contamination of miscellaneous bacteria. Not suitable for large-scale application

However, there are the following points to be improved: 1) there is no description of the selected position of the axillary buds, because the incidence rates of aseptic buds formed by the axillary buds at different stem nodes are different, and the too tender axillary buds will lose chlorosis and die under the treatment of mercuric chloride, which has no effect. Bacterial germination rate; 2) We used the method of Yin Liqing and Zheng Guichao to sterilize the aseptic buds, and found that the pollution rate was above 80%, and the germination was slow, so it is necessary to optimize this technical link; 3) Aseptic bud proliferation The coefficient is low and needs to be improved

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