A kind of inoculation identification method of soybean resistance to whitefly virus disease

An identification method and technology of Bemisia tabaci, applied in the field of agricultural science, can solve the problems of agricultural production loss, the identification results are easily affected by the environment, and the scope of application is limited.

Active Publication Date: 2016-12-14
抚远市黑瞎岛生态物流有限责任公司
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

If soybeans with a strong tendency to bemisia tabaci are used as a source of resistance to breed bemisia tabaci virus disease-resistant varieties, large-scale planting will cause bemisia tabaci to be forced to choose to feed the virus, and finally make soybeans resistant to bemisia tabaci virus disease through the field. The disappearance of the false disease resistance manifested by natural disease identification may bring greater losses to agricultural production
[0010] Although the field natural disease investigation method can compare the differences in the traits of Bemisia tabaci among soybean varieties, the identification results are easily affected by the environment, and the scope of application is also limited, and it is impossible to rule out the interference of row tropism, which leads to insufficient identification results precise

Method used

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  • A kind of inoculation identification method of soybean resistance to whitefly virus disease
  • A kind of inoculation identification method of soybean resistance to whitefly virus disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] On the night of August 5, 2013, an insect trap lamp was installed in the field to trap live adults of Bemisia tabaci. After one night of trapping, a large number of Bemisia tabaci were collected in the accumulation bottle, which also contained a large number of miscellaneous insects. The purification and screening device for planthopper adults (ZL 201310290925.X) was used for separation and screening. In order to separate pure whitefly adults with uniform ages, a combination of sampling sieves with different apertures was tested and counted by visual observation.

[0037]

[0038] The screening investigation found that the combination of 45-55 mesh sub-sampling sieves can effectively screen out the dead insects of Bemisia tabaci, impurities and no (weak) phototaxis insects, large phototaxis insects, small phototaxis insects and juvenile Bemisia tabaci nymphs. Interference, effective isolation of uniform whitefly adults.

Embodiment 2

[0040] 1. The difference between using this technology to identify soybean yellow mosaic disease and identifying soybean yellow mosaic disease naturally occurring in the field

[0041] In 2013, the field natural disease identification method was used to investigate the incidence of soybean yellow mosaic disease in several severely affected areas of soybean bemisia tabaci, and at the same time, this technology was used to identify the incidence of soybean yellow mosaic disease.

[0042] Analyzing Table 2, it was found that there was a significant difference in the incidence of soybean yellow mosaic disease between the five soybean varieties identified by this method and the natural disease identification in the hardest-hit areas, and the relative resistance-sensitivity ratios of the other four varieties relative to the incidence of yellow mosaic disease in Ludou No. 10 were also different. Mostly significant, Huapidou and Langziwei's yellow disease incidence relative to resistan...

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Abstract

The invention discloses a method for indoor inoculating and identifying resistance of soybeans for a virus disease of Bemisia tabaci. The method comprises the steps that 1, the large field Bemisia tabaci is obtained, and then a separation and purification are conducted on a plurality of regular Bemisia tabaci imagoes; 2, a plurality of regular third instar Bemisia tabaci nymphs are bred, separated and selected; 3, poison is fed to the obtained third instar Bemisia tabaci nymphs; 4, the Bemisia tabaci nymphs are measured; (e) a to-be-detected soybean variety and the poison third instar Bemisia tabaci nymphs are rationed, fed together and transmitted poison; 5, symptomatic appearance of the large field is counted: the to-be-detected soybeans are transplanted to a large field insect separating hypocaust, investigation and statistics are conducted on morbidity of diseased plants of a soybean Bemisia tabaci virus after the symptomatic appearance of the large field is conducted, and the disease resistance ability of the to-be-detected soybean variety can be accurately identified. According to the method for indoor inoculating and identifying, the interference of antixenosis and the problem of the obtained scale volume of the Bemisia tabaci are overcome, the inoculation and identification of the ration and reinforcement of the soybean Bemisia tabaci virus disease are achieved, and the resistance level of the soybean variety for the soybean Bemisia tabaci virus disease can be accurately evaluated.

Description

technical field [0001] The invention relates to an inoculation identification method of soybean resistance to bemisia tabaci virus disease, belonging to the field of agricultural science and technology. Background technique [0002] Bemisia tabaci, belonging to the order Homoptera, Bemisidae, genus Bemisia, was first reported in 1889 and found on tobacco in Greece, named as Bemisia tabaci. In 1894, the first Bemisia tabaci in the New North District was found on sweet potatoes in Florida, USA, and was identified as Bemisia tabaci. Because of its morphological variability, Bemisia tabaci has produced many synonyms. Therefore, Bemisia tabaci is also known as whitefly and sweet potato whitefly. By 1978, the number of synonyms of Bemisia tabaci had reached 22. Bemisia tabaci has a variety of feeding habits and a wide range of host plants. There are more than 420 species of host plants in 74 families. [0003] Bemisia tabaci sucks the sap of host plants with piercing-sucking mo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G7/00A01K67/033
CPCA01G7/00A01K67/033
Inventor 许伟
Owner 抚远市黑瞎岛生态物流有限责任公司
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