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A seedling cultivation and transplanting method for improving the root system of sea cauliflower tissue culture seedlings

A technology for tissue culture seedlings and sea cauliflower, applied in the directions of botanical equipment and methods, horticultural methods, cultivation, etc., can solve the problems of inability to penetrate non-woven seedling bags, hinder the popularization and application of sea cauliflower tissue culture seedlings, etc., and achieve protection Diversity of ecological environment, improving the survival rate of transplanting, and the effect of strong penetrating power

Active Publication Date: 2016-04-27
广西壮族自治区农业科学院生物技术研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the root system of sea cauliflower tissue culture seedlings is relatively fragile and cannot penetrate the non-woven fabric seedling bag, which seriously hinders the popularization and application of sea cauliflower tissue culture seedlings.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A seedling cultivation and transplanting method for improving the root system of sea cauliflower tissue culture seedlings, comprising the following steps:

[0024] 1. On the ultra-clean workbench, cut the 3-7cm-high aseptic buds obtained from the multiplication and cultivation of sea cauliflower into single buds, insert them into the rooting medium, and cultivate them in the cultivation room for 30 days. The root length is about 0.5cm , to obtain sea cauliflower tissue culture seedlings; the rooting medium is: MS+KT0.05mg / L+6-BA0.05mg / L+NAA0.1mg / L+activated carbon 1.0g / L+sucrose 20g / L+agar 5g / L, The pH value is 5.8; the culture conditions in the culture room are: culture temperature is 25±3°C, light time is 12h / d, light intensity is 1500lx.

[0025] 2. Take out the sea cauliflower tissue culture seedlings from step 1 and move them out of the cultivation room, harden the seedlings indoors for 3 days, take the seedlings, carefully wash the medium attached to the roots, pu...

Embodiment 2

[0028] A seedling cultivation and transplanting method for improving the root system of sea cauliflower tissue culture seedlings, comprising the following steps:

[0029] 1. On the ultra-clean workbench, cut the 3-7cm high aseptic buds obtained from the multiplication and cultivation of sea cauliflower into single buds, insert them into the rooting medium, and cultivate them in the cultivation room for 20 days. The root length is about 0.5cm , to obtain sea cauliflower tissue culture seedlings. The rooting medium is: MS+KT0.5mg / L+6-BA0.5mg / L+NAA3.0mg / L+activated carbon 1.0g / L+sucrose 20g / L+agar 5g / L, the pH value is 5.8; The culture conditions in the room are: the culture temperature is 25±3℃, the light time is 12h / d, and the light intensity is 1500lx.

[0030] 2. Take out the sea cauliflower tissue culture seedlings from step 1 and move them out of the cultivation room, harden the seedlings indoors for 3 days, take the seedlings, carefully wash the medium attached to the roo...

Embodiment 3

[0033] A seedling cultivation and transplanting method for improving the root system of sea cauliflower tissue culture seedlings, comprising the following steps:

[0034] 1. On the ultra-clean workbench, cut the 3-7cm-high aseptic buds obtained from the multiplication and cultivation of sea cauliflower into single buds, insert them into the rooting medium, and cultivate them in the cultivation room for 15 days. The root length is about 0.5cm , to obtain sea cauliflower tissue culture seedlings. The rooting medium is: MS+KT0.1mg / L+6-BA0.1mg / L+NAA1.0mg / L+activated carbon 1.0g / L+sucrose 20g / L+agar 5g / L, the pH value is 5.8; The culture conditions in the room are: the culture temperature is 25±3℃, the light time is 12h / d, and the light intensity is 1500lx.

[0035] 2. Take out the sea cauliflower tissue culture seedlings from step 1 and move them out of the cultivation room, harden the seedlings indoors for 3 days, take the seedlings, carefully wash the medium attached to the roo...

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Abstract

The invention discloses a seedling culture and transplanting method of an improved Ottelia acuminata tissue culture seedling root system. The method comprises the three steps of the rooting culture of tissue culture seedlings, the improvement of the Ottelia acuminata tissue culture seedling root system and the heeling-in and transplanting of the Ottelia acuminata tissue culture seedlings. The method has the beneficial effects that: 1. the rooting time of the Ottelia acuminata tissue culture seedlings can be shortened, the tissue culture seedlings are healthy, the root system is developed, the flexibility of roots is good, and the penetrating power of root tips is strong, so that the Ottelia acuminata tissue culture seedling root system can be tightly combined with a non-woven bag and a medium thereof and is directly put into lakes and rivers in which the Ottelia acuminata needs to be planted, and thus the transplanting survival rate of the tissue culture seedlings is greatly improved; 2. the operation is simple, the production cost is low, the seedlings can be cultured and transplanted on a large scale, a technical support is provided for promoting and applying the Ottelia acuminata tissue culture seedlings, and a foundation is laid for the sustainable utilization of the Ottelia acuminata and the protection of ecological environment diversity.

Description

technical field [0001] The invention relates to the technical field of plant vegetative propagation, in particular to a seedling cultivation and transplanting method for improving the root system of sea cauliflower tissue culture seedlings. Background technique [0002] Sea cauliflower [Ottelia acuminata (Gagnep.) Dandy], also known as sea vegetable and dragon claw dish, belongs to the perennial aquatic plant of Hydrocharidaceae. Sea cauliflower is a unique species in my country, mainly distributed in Yunnan, Guizhou, Guangxi and parts of Hainan. Sea cauliflower can grow in lakes, ponds, ditches and deep water fields, and the water body is required to be clean, clear and transparent, and it likes warmth. The general flowering period is from May to October, and there are flowers all year round in warm areas. [0003] Sea cauliflower is a submerged plant integrating ornamental, edible, medicinal and slight water pollution purification. The yellow pistils and white petals of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G31/00
Inventor 韦绍龙李小泉其他发明人请求不公开姓名
Owner 广西壮族自治区农业科学院生物技术研究所
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