Kit and method for rapidly extracting blood DNA in batches by adopting saturated sodium chloride process

Abstract

The invention provides a kit and a method for rapidly extracting blood DNA in batches by adopting a saturated sodium chloride process. The kit contains red blood cell lysate, a deproteinization solution, a protein precipitation solution, a regulation binding solution, an eluant and a proteinase k solution, wherein the red blood cell lysate contains 8.29g / L NH4Cl, 31g / L KHCO3 and 0.37g / L Na2EDTA, and pH value is 7.2-7.4; the deproteinization solution is 20% SDS; the protein precipitation solution is saturated NaCl; the regulation binding solution is isopropyl alcohol; the eluant is 70% ethyl alcohol; and concentration of the proteinase k solution is 20mg / ml. The method for extracting the blood DNA by utilizing the kit has the advantages that content of the extracted DNA is high, purity is high, extraction is rapid, no toxic reagent is adopted, PCR amplification detection is carried out on the extracted genome DNA, and the extracted blood DNA can meet the requirements of many follow-up molecular biology experiments.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a kit for rapidly extracting blood DNA by a saturated sodium chloride method, and also relates to a method for using the kit to extract DNA from blood samples. Background technique [0002] The genomic DNA extracted from different sources of material is very different due to the difference in the starting amount of the material, the cell structure and its components. Therefore, sufficient material input is critical to extract enough genomic DNA for subsequent experiments. In many zoological experiments, a large amount of genomic DNA can be obtained by collecting a small amount of animal blood, and the collection method is fast, so that a lot of molecular genetic breeding research can be carried out. Therefore, the effective extraction of genomic DNA from blood samples becomes a key step to achieve the goals in the above fields. [0003] At present, there are various methods for...

AI Technical Summary

Problems solved by technology

Although the spin column method can extract DNA and ensure its purity, the yield of DNA extracted by it is generally not high, which cannot meet the requirements of large-scale animal molecular genetic analysis, forensic testing, archaeological analysis, and medical testing.

In addition, the spin column method uses some toxic reagents, such as phenol, chloroform, etc., which is not conducive to the health of the experimenters; at the same time, the spin column method consumes more time in the whole process of extracting DNA, which affects the speed of the next analysis and detection.

[0004] As a relatively safe and effective method for extracting genomic DNA from blood using the saturated sodium chloride method, there is no special kit, so we invented this kit based on the method of removing protein by the saturated sodium chloride method to quickly extract blood DNA

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