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Rooting culture method for tamarix chinensis tissue culture seedlings

A technology of rooting culture and tissue culture seedlings, which is applied in the field of plant cultivation, can solve problems such as the influence of proliferation speed and rooting rate, unsuitability for large-scale production, and browning of the base of tissue culture seedlings, so as to shorten the cultivation cycle and reduce material costs , to ensure the effect of genetic traits

Inactive Publication Date: 2015-04-29
上海杉一植物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This phenomenon did not appear in the explant induction process, but it would occur in the subculture process and after being transferred to the rooting medium. The tissue culture seedlings with browning phenomenon could still grow and take root, but the proliferation rate and rooting In severe cases, the base of tissue culture seedlings will gradually brown and endanger their survival
Since the browning phenomenon only occurs in a small number of tissue culture seedlings, and has a certain relationship with the physiological state of the explant material, it is necessary to select young and vigorous explants as much as possible when selecting explant materials. The old part needs to be discarded, and only the new part is subcultured, which can reduce part of the browning phenomenon, but the number of tissue cultured seedlings cultivated by this operation is small, which is not suitable for large-scale production
[0005] A small amount of browning seedlings have been reported in the prior art, only need to replace the new medium after browning or move the seedlings to different parts of the same medium, the browning phenomenon is basically solved, and the browning is serious after replacing two Browning no longer occurs after subculture, but this operation not only increases the operation steps, but also increases the production cost

Method used

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  • Rooting culture method for tamarix chinensis tissue culture seedlings
  • Rooting culture method for tamarix chinensis tissue culture seedlings
  • Rooting culture method for tamarix chinensis tissue culture seedlings

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Embodiment 1

[0023] Embodiment 1 A kind of method of Tamarix tissue culture seedling rooting culture

[0024] A method for rooting culture of tamarix tissue cultured seedlings, it is carried out according to the following steps:

[0025] (1) Acquisition of sterile vaccines

[0026] Take new shoots or buds as explants, soak them in washing powder water twice, and then rinse them with water for 30 minutes; then disinfect them with 70% alcohol for 20 seconds, rinse them twice with sterile water, and then rinse them with 0.1% mercuric chloride 100ml and Disinfect with 0.05ml Tween-20 for 10 minutes, and finally wash 6 times with sterile water in the ultra-clean workbench to remove the water on the surface of the explants, trim them, and transfer the trimmed explants to medium A for Cultivation, the culture conditions are: light culture for 13 hours, the light intensity is 2000 lux, and the temperature is 25°C; after 20 days of cultivation, the sterile seedlings are obtained;

[0027] (2) Pro...

Embodiment 2-6

[0033] The method that embodiment 2-6 Tamarix tissue culture seedling takes root culture

[0034] Embodiment 2-6 is respectively a kind of method of tamarix tissue culture seedling rooting culture, and the cultivation method of embodiment 1 is similar, and difference only is wherein involved technical parameter and the index of made tamarix tissue culture seedling different, as shown in the table below:

[0035]

[0036]

[0037] The technical parameter of embodiment 2-6 subculture, as shown in the following table:

[0038]

Embodiment 7

[0039] The screening method of embodiment 7 medium A

[0040] Conditional screening has been carried out on medium A. With MS as the base medium, sucrose 20g / L, agar 5.8g / L, and pH adjusted to 6.5, different types of nutrients or additives were added thereto. For the Tamarix group The growth status of the cultured seedlings has obvious differences, especially while reducing the brown matter, the basic requirements of the tissue cultured seedlings (such as plant height, root length and root quantity, etc.) are guaranteed. The specific process is shown in the following table:

[0041]

[0042]

[0043] It can be seen from the above table that after adding activated carbon components, observing the growth of tamarisk tissue culture seedlings, the appearance of brown matter is greatly reduced, and the rooting condition of tissue culture seedlings is good, which meets the requirements for continued subculture and direct greenhouse planting .

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Abstract

The invention discloses a subculture method of tamarix chinensis tissue culture seedlings. The subculture method is a one-step seedling culturing method. According to the method, an active carbon component is added into a culture medium, the phenomenon that a tamarix chinensis explants is browned is reduced, the culture period is shortened, the consumption of sugar is reduced, the production cost is reduced, the material cost is further reduced, the stability of inheritable characters of the tamarix chinensis are ensured, a large quantity of high-quality tamarix chinensis tissue culture seedlings are obtained in a short period, and an important significance to the industrialization of the tissue culture of the tamarix chinensis is exerted. The method is applicable to rooting culture of the tamarix chinensis tissue culture seedlings and is further applicable to the subculture of seedlings planted in greenhouses or the tissue culture seedlings.

Description

technical field [0001] The invention belongs to the field of plant cultivation, and relates to a method for cultivating plants, in particular to a method for rooting and cultivating tamarix tissue cultured seedlings. Background technique [0002] Tamarix is ​​a saline woody plant with excellent characteristics such as high salt-alkali tolerance, severe cold resistance, drought resistance, and water-humidity resistance. It can survive and is the first choice tree species for coastal shelterbelts and severe saline-alkali land afforestation. Chinese Tamarix also has a strong salt-excreting ability and a well-developed root system, which can excrete absorbed salt through the leaves, play a role in windbreak and sand fixation, and soil improvement. Tamarix with excellent characteristics is of great significance to the improvement of saline-alkali land, greening of saline-alkali land, construction of coastal shelter forest and ecological improvement of saline-alkali land. In add...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 刘伯燕王苏洋刘戈陈娜林大为张承妹董举文陈铭高畅苏涌
Owner 上海杉一植物科技有限公司
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