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Suaeda salsa saline-alkaline tolerance gene and application thereof

A technology of Suaeda salsa and salt-alkali tolerance, which is applied in the field of molecular biology and biology, and can solve problems such as lack of understanding of molecular mechanisms and obstacles to the cultivation of salt-tolerant crops

Inactive Publication Date: 2015-04-22
TIANJIN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The creation of salt-tolerant crop varieties is one of the important means to achieve stable and sustainable growth of food production under the background of global industrialization. However, the lack of understanding of the molecular mechanism of crop salt tolerance and genes related to salt tolerance hinders the cultivation of salt-tolerant crops

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  • Suaeda salsa saline-alkaline tolerance gene and application thereof
  • Suaeda salsa saline-alkaline tolerance gene and application thereof
  • Suaeda salsa saline-alkaline tolerance gene and application thereof

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Embodiment Construction

[0016] The technical solutions of the present invention will be further described below in conjunction with specific embodiments.

[0017] The source of Suaeda salsa (Suaeda salsa) used is Donghai Road, Beitangkou, Binhai New Area, Tianjin.

[0018] First, use this as a raw material to obtain the saline-alkali tolerance gene (that is, the clone of Suaeda salsa SAT gene) of the present invention.

[0019] Take five-week-old fresh Suaeda salsa plants, use the plant EasyPure PCR Purification Kit (Trans gene Code #EP101-0150rxns) to extract total RNA, and use EasyScript Frist-Strand cDNA SynSgesis SuperMix (Trans gene Code #AE301-03100rxns) to remove genomic DNA Interference, cDNA was reverse transcribed.

[0020] According to the sequence conservation region of the Arabidopsis thaliana SAT32s gene in the NCBI database, degenerate primers SAT-5 and SAT-3 were designed, and then using RACE-PCR technology (Introgen, RLM-RACE Kit with Manual) to obtain the full-length cDNA sequenc...

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Abstract

The invention discloses a suaeda salsa saline-alkaline tolerance gene and application thereof. A nucleotide as shown in the SEQ ID NO.1 in a sequence table is acquired from suaeda salsa, and a recombinant vector and a host cell containing the gene can also be acquired; and arabidopsis and poplar transfected by the gene show tolerance on saline-alkaline, which indicates the application of the saline-alkaline tolerance gene provided by the invention in improvement of saline-tolerance tolerance of crops.

Description

technical field [0001] The invention belongs to the fields of molecular biology and biotechnology, and more specifically relates to a saline-alkali-resistant gene selected from Suaeda salsa and its application. Background technique [0002] Soil salinization is one of the serious problems facing the world's agricultural production, and salt stress is an important factor limiting plant growth and development. With the development and utilization of saline-alkali soil in recent years, the country has paid more and more attention to the isolation and function of salt-resistant genes, which has become a hot research direction in biology. The creation of salt-tolerant crop varieties is one of the important means to achieve stable and sustainable growth of food production under the background of global industrialization. However, the lack of understanding of the molecular mechanism of crop salt tolerance and genes related to salt tolerance hinders the cultivation of salt-tolerant ...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/82C12N1/21A01H5/00
Inventor 王洁华罗翠杨少辉
Owner TIANJIN UNIV
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