EPS8-derived antitumor CTL epitope peptide and application thereof

An epitope peptide and anti-tumor technology, applied in the field of biochemistry, can solve problems such as unsatisfactory tumor treatment effects, achieve the effect of preventing recurrence and good application prospects

Active Publication Date: 2015-04-08
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional treatment methods such as surgery, radiotherapy and chemotherapy are not effective for some tumors, especially advanced malignant tumors.

Method used

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  • EPS8-derived antitumor CTL epitope peptide and application thereof
  • EPS8-derived antitumor CTL epitope peptide and application thereof
  • EPS8-derived antitumor CTL epitope peptide and application thereof

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Embodiment Construction

[0033] In the following, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.

[0034] 1. Prediction of EPS8HLA-A*0201 restricted CTL epitope

[0035] 1. Prediction of CTL epitopes by biological software

[0036] The EPS8-derived anti-tumor CTL epitope peptide analog of the present invention is based on the primary structure of the antigen, using immunoinformatics means, and using the online biological software SYFPEITHI, BIMAS and NetMHC 3.2 to analyze the HLA-A of the EPS8 antigen structure *0201 Restrictive predictive analysis, screening WTQDMILQV (denoted as EPS8-101), ILDDIEFFI (denoted as EPS8-276), FLFTPLNMV (denoted as EPS8-360) and QLAESVANV (denoted as EPS8-455) as candidate CTL epitope peptides , and according to the influence of different amino acid residues on the HLA-A*0201 restricted epitope motif and other positions on the affinity of the epitope and HLA-A*0201 molecules, the main anchoring res...

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Abstract

The invention discloses an EPS8-derived antitumor CTL epitope peptide. The EPS8-derived antitumor CTL epitope peptide is a nonapeptide with the sequence of Phe-Leu-Phe-Thr-Pro-Leu-Asn-Met-Val. The invention also discloses an application of the epitope peptide to a drug. A specific CTL induced by the EPS8-derived antitumor CTL epitope peptide can secrete a certain IFN-gamma and has a certain antigen-specificity killing effect for HLA-A*0201, EPS8 positive-expression MCF-7 cells and EPS8-loaded T2A2 cells; and a prepared EPS8 positive-expression tumor treating polypeptide vaccine has huge development and application potentials in the field of specific immunotherapy of tumor.

Description

[0001] This application is a divisional application of a Chinese invention patent application with an application date of December 23, 2013, an application number of 201310717092.0, and an invention title of EPS8 anti-tumor CTL epitope peptide and its application. technical field [0002] The invention belongs to the technical field of polypeptides in the field of biochemistry, and in particular relates to an anti-tumor CTL epitope peptide derived from EPS8 and its application in preparing medicines for treating tumors. Background technique [0003] Malignant tumor is the first killer of human beings in the new century. Traditional treatment methods such as surgery, radiotherapy and chemotherapy have not been satisfactory for some tumors, especially advanced malignant tumors. In recent years, the role of Cytotoxic T Lymphocytes (CTL) in suppressing tumors has received great attention. How to effectively stimulate the specific cellular immune response mediated by CTLs and exe...

Claims

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Application Information

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IPC IPC(8): C07K14/47A61K39/00A61P35/00
CPCA61K39/00C07K14/4748
Inventor 李玉华周炜均
Owner SOUTHERN MEDICAL UNIVERSITY
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