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Method for measuring content of vitamin K2 (MK-7)

A method of determination, technology of vitamins, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of shortening the service life of chromatographic columns, low work efficiency, damage to chromatographic columns, etc., and achieve short test time, easy operation and low detection cost low effect

Active Publication Date: 2015-03-25
JIANGSU ALAND NOURISHMENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows for accurate analysis by controlling its temperatures during different stages such that no harmful substances are released from certain materials while maintain their effectiveness over longer periods. Additionally, this technology provides quicker testing times with easy-to use instruments and lower costs compared to current methods like liquid phase extraction techniques. Overall, these technical improvements improve the overall performance of analyzers and make them more efficient than older types of equipment.

Problems solved by technology

Technic problem addressed in this patented study relates to accurately detecting or measuring certain substances called vitamin ketones that can help improve bone health without causing harmful effects on other parts of our bodies such as skin cells.

Method used

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  • Method for measuring content of vitamin K2 (MK-7)
  • Method for measuring content of vitamin K2 (MK-7)
  • Method for measuring content of vitamin K2 (MK-7)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1. Detection reagent

[0039] 1.1 Vitamin K 2 (MK-7) Reference Substance (USP)

[0040] 1.2 Isopropanol (HPLC)

[0041] 1.3 Acetonitrile (HPLC)

[0042] 1.4 Absolute ethanol (HPLC)

[0043] 2. Detection

[0044] 2.1 Reference substance solution preparation

[0045] Accurately weigh 10mg vitamin K 2 (MK-7) Add 30ml of isopropanol to the reference substance in a 50ml volumetric flask, ultrasonically dissolve the powder, and dilute to the mark with isopropanol as a stock solution. Dilute the stock solution with isopropanol to make reference substance solutions containing 1.0 μg, 2.0 μg, 4.0 μg, 6.0 μg, and 8.0 μg per 1 ml, and store them in a cool and dark place.

[0046] 2.2 Preparation of sample solution

[0047] 2.2.1 Weigh 3.0g of powder sample (product name: calcium powder, batch number: YB1402601, manufacturer: Jiangsu Alande Nutritional Products Co., Ltd.) into a 50ml brown volumetric flask, add 30ml of isopropanol, and place in a constant temperature wat...

Embodiment 2

[0063] 1. Detection reagent

[0064] 1.1 Vitamin K 2 (MK-7) Reference Substance (USP)

[0065] 1.2 Isopropanol (HPLC)

[0066] 1.3 Acetonitrile (HPLC)

[0067] 1.4 Absolute ethanol (HPLC)

[0068] 2. Detection

[0069] 2.1 Reference substance solution preparation

[0070] Accurately weigh 10mg vitamin K 2 (MK-7) Add 30ml of isopropanol to the reference substance in a 50ml volumetric flask, ultrasonically dissolve the powder, and dilute to the mark with isopropanol as a stock solution. Dilute the stock solution with isopropanol to make reference substance solutions containing 1.0 μg, 2.0 μg, 4.0 μg, 6.0 μg, and 8.0 μg per 1 ml, and store them in a cool and dark place.

[0071] 2.2 Preparation of sample solution

[0072] 2.2.1 Take the contents of 20 soft capsules (product name: vitamin KD calcium soft capsules, batch number: YB1400201, production unit: Jiangsu Alande Nutrition Co., Ltd.), mix them evenly, and accurately weigh 5.0g of the sample into a 50ml brown volu...

Embodiment 3

[0088] 1. Detection reagent

[0089] 1.1 Vitamin K 2 (MK-7) Reference Substance (USP)

[0090] 1.2 Isopropanol (HPLC)

[0091] 1.3 Acetonitrile (HPLC)

[0092] 1.4 Absolute ethanol (HPLC)

[0093] 2. Detection

[0094] 2.1 Reference substance solution preparation

[0095] Accurately weigh 10mg vitamin K 2 (MK-7) Add 30ml of isopropanol to the reference substance in a 50ml volumetric flask, ultrasonically dissolve the powder, and dilute to the mark with isopropanol as a stock solution. Dilute the stock solution with isopropanol to make reference substance solutions containing 1.0 μg, 2.0 μg, 4.0 μg, 6.0 μg, and 8.0 μg per 1 ml, and store them in a cool and dark place.

[0096] 2.2 Preparation of sample solution

[0097] 2.2.1 Take 20 tablets (product name: vitamin KD calcium chewable tablets, batch number: YB1401001, production unit: Jiangsu Alande Nutritional Products Co., Ltd.), crush them, mix them evenly, and accurately weigh 5.0g of the sample in a 50ml brown ca...

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Abstract

The invention discloses a method for measuring content of vitamin K2 (MK-7) and mainly relates to the sample extraction technology and chromatographic separation technology of the vitamin K2 (MK-7). The content measuring method comprises the following steps: (1) preparing a sample solution; (2) measuring the content of the vitamin K2 (MK-7), namely measuring the sample solution by utilizing a high performance liquid chromatography technology to obtain the peak area of a chromatographic peak of the vitamin K2 (MK-7), calculating the content of the vitamin K2 (MK-7) in a tested sample according to a standard curve, and calculating in combination with the mass of the sample to obtain the content of the vitamin K2 (MK-7). The method can be used for rapidly measuring the content of the vitamin K2 (MK-7) contained in health foods, medicines, common foods or premix, the quality of the health foods, medicines, common foods or premix containing the vitamin K2 (MK-7) is evaluated, and the content measuring method is accurate, rapid, convenient and effective.

Description

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Claims

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Application Information

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Owner JIANGSU ALAND NOURISHMENT
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