A kind of method for extracting anthocyanin from raspberry
A technology of raspberry anthocyanin and extraction method, applied in the direction of organic chemistry and the like, can solve the problems of unpublished extraction of anthocyanin and the like, and achieve the effects of convenient and rapid activation, high purity and few impurities
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Embodiment 1
[0008] Take 1 kg of washed fresh berry fruit and beat it into a homogenate with a homogenizer. Take 3L of ethanol, 0.5L of acetic acid, and 1.5L of water and mix them to obtain the extractant. After fully mixing the berry homogenate with the extractant, perform ultrasonic extraction at 45°C for 90 minutes, and then vacuum filter the extract to obtain anthocyanins Crude extract, the crude extract was rotary evaporated to no alcohol smell. Use 2000mL of XAD7 macroporous resin for adsorption, the flow rate is controlled at 0.5BV / h, first use distilled water for elution, the elution volume is 4L, and then use 95% ethanol for elution, the elution volume is 10L. Obtain the eluate of anthocyanins from the old berry. The anthocyanin eluate from the old berry was treated by ultra-high pressure equipment at 700Mpa and 40°C for 30 minutes, and then the ethanol was removed by vacuum rotary evaporation, extracted with 1.5L ethyl acetate, and the organic phase was removed after standing fo...
Embodiment 2
[0010] Take 2 kg of washed fresh berry fruit and beat it into a homogenate with a homogenizer. Take 6L of ethanol, 1L of acetic acid, and 3L of water and mix them to obtain the extractant. After fully mixing the berry homogenate with the extractant, perform ultrasonic extraction at 45°C for 90 minutes, and then vacuum filter the extract to obtain crude anthocyanins. solution, and the crude extract was rotary evaporated until it had no alcohol smell. Use 4000mL of XAD7 macroporous resin for adsorption, the flow rate is controlled at 0.5BV / h, first use distilled water for elution, the elution volume is 12L, and then use 95% ethanol for elution, the elution volume is 24L. Obtain the eluate of anthocyanins from the old berry. The anthocyanin eluate from the old berry was treated by ultra-high pressure equipment at 700Mpa and 40°C for 30 minutes, then the ethanol was removed by vacuum rotary evaporation, extracted with 9.5L ethyl acetate, and the organic phase was removed after st...
Embodiment 3
[0012] Take 5 kg of washed fresh berry fruit and beat it into a homogenate with a homogenizer. Take 15L of ethanol, 2.5L of acetic acid, and 7.5L of water and mix them to obtain the extractant. After fully mixing the berry homogenate with the extractant, perform ultrasonic extraction at 45°C for 90 minutes, and then vacuum filter the extract to obtain anthocyanins Crude extract, the crude extract was rotary evaporated to no alcohol smell. Use 10000mL of XAD7 macroporous resin for adsorption, the flow rate is controlled at 0.5BV / h, first use distilled water for elution, the elution volume is 20L, and then use 85% ethanol for elution, the elution volume is 50L. Obtain the eluate of anthocyanins from the old berry. Treat the anthocyanin eluent of the old berry with ultra-high pressure equipment at 700Mpa and 45°C for 30 minutes, then remove the ethanol by vacuum rotary evaporation, extract with 10L ethyl acetate, remove the organic phase after standing for stratification, and co...
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