A kind of method and application of cross pollination of celery

A technology for hybrid pollination and celery, applied in the directions of application, botanical equipment and method, plant genetic improvement, etc., can solve the problems of lack of cost, moderate pollination and isolation methods, celery seed strains are difficult to achieve pollen isolation, etc., and achieve low cost. , the effect of preventing the mixing of varieties

Active Publication Date: 2016-08-03
TIANJIN RES INST OF VEGETABLE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Nylon mesh bags have good permeability and can overcome the above problems. However, even the 80-mesh mesh with the largest mesh number on the agricultural materials market is difficult to achieve 100% pollen isolation for high-density planted celery plants in commercial breeding.
my country's celery breeding research started late, and the overall technical level obviously lags behind other vegetable crops. One of the key problems is the lack of an efficient, practical and cost-effective pollination and isolation method

Method used

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  • A kind of method and application of cross pollination of celery
  • A kind of method and application of cross pollination of celery

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Experimental program
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Effect test

Embodiment 1

[0036] The celery first-generation hybrid variety "Jinrui 1" with CMS traits was used to cross with the target transgenic material "Ventura", and F1 was backcrossed with "Ventura" for 4 consecutive generations to obtain the target CMS male sterile line female. This "C01-3A", "Ventura" became the corresponding maintainer line "C01-3B" of the CMS female parent "C01-3A" after selfing.

[0037] In spring, select the strong celery CMS female parent "C01-3A" and male parent "Qianfang" that have passed vernalization, remove the peripheral old leaves and the upper half of the petiole respectively, and prepare them with 1 million units of veterinary streptomycin Soak the roots with 1000 times the solution, and then plant the CMS female parent and male parent plants in pairs at a distance of 0.5 meters, and keep a distance of 0.8 meters between each pair of plants and adjacent plants. Immediately insert a field recording sign next to each pair of seed plants, step on the topsoil of the ...

Embodiment 2

[0042] The first-generation celery hybrid variety "Kuai Nen 60" with CMS traits was used to cross with the target transgenic material "Youventus", and F1 was backcrossed with "Youventus" for 4 consecutive generations to obtain the target CMS male sterile line The female parent "C10-80A" and "Youventus" became the corresponding maintainer line "C10-80B" of the CMS female parent "C10-80A" after selfing.

[0043] In spring, select the strong celery CMS female parent "C10-80A" and male parent "California Emperor" strains that have passed vernalization, remove the peripheral old leaves and the upper half of the petiole, and prepare them with 1 million units of veterinary streptomycin 1000 times the solution was soaked in the roots, and then the CMS female parent and male parent plants were planted in pairs at a distance of 0.5 meters, and a distance of 0.6 meters was maintained between each pair of plants and adjacent plants. Immediately insert a field recording sign next to each p...

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Abstract

The invention discloses a hybrid pollination method of celery and application of the method. The hybrid pollination method of celery comprises the following steps: firstly, carrying out transform breeding to obtain cytoplasmic male sterility (CMS for abbreviation) female parents and corresponding maintenance lines of the celery, selecting vernalized strong seed plants of CMS female parents and male parents to plant in pairs, arranging isolation net bags before bolting until flowering, carrying out artificial supplementary pollination in the flowering period, and ensuring that the CMS female parents in a hybridized combination only can obtain effective pollination of the male parents. The invention aims to ensure that the CMS female parent plants in the celery hybridized combination can obtain the effective pollination of the male parents; 100% pollen isolation between different combinations is implemented; the varietal complexity caused by mixture of pollen can be avoided; the breeding efficiency is improved.

Description

technical field [0001] The invention belongs to the technical field of agricultural vegetable planting, and relates to a method and application of cross pollination of celery. Background technique [0002] Celery is one of the important vegetable crops in my country. It belongs to the genus Celery of Umbelliferae. It is a biennial vegetable with entomophilous flowers. It is often cross-pollinated. The flowers are small and large, and the flower branches are soft and overlapping. The flowering period is long and scattered. It is very difficult to carry out strict cross-pollination artificially. Big. In the past, in order to ensure the purity of a variety, it was generally necessary to keep a natural isolation distance of more than 1,000 meters from other varieties during seed production. However, this approach is far from meeting the needs of modern commercial breeding work, because commercial breeding must follow the principle of high efficiency and precision, there are a lo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02
Inventor 高国训王武台吴锋刘惠静
Owner TIANJIN RES INST OF VEGETABLE
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