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Biological sample spectral analysis method based on solute migration electrospray ionization technique

A technology of electrospray ionization and biological samples, which is applied in the field of mass spectrometry ionization source, can solve the problems of unfavorable mass spectrometry analysis sensitivity, inability to effectively avoid sample matrix interference, increase and other problems, and achieve the effect of no need for sample pretreatment, simple operation and high analysis sensitivity

Inactive Publication Date: 2015-01-28
XI'AN PETROLEUM UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these methods play a key role in the rapid analysis of small-volume complex samples, they cannot effectively avoid the interference of the sample matrix, which is not conducive to the improvement of the sensitivity of mass spectrometry.

Method used

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  • Biological sample spectral analysis method based on solute migration electrospray ionization technique
  • Biological sample spectral analysis method based on solute migration electrospray ionization technique
  • Biological sample spectral analysis method based on solute migration electrospray ionization technique

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The solute transport electrospray ionization source loads the analytical sample at the end of the solvent

[0024] At room temperature, inject 20 μL of solvent (methanol) into a capillary with a tip pore size of 20 μm, and then use a pipette to load 2 μL of blood sample containing the target drug compound on the end of the solvent. When a DC voltage of 2.0kV is applied, the drug compound in the blood sample will be extracted by the solvent, and migrate to the tip of the capillary, and then the electrospray behavior will occur. In this process, the matrix of the blood sample will not change with the migration of the solvent. The specific process is as follows:figure 1 shown. figure 1 A is the situation of the electrospray capillary after adding 2 μL blood sample; figure 1 B is the picture taken by the capillary after applying a 2.0kV DC voltage to the spray capillary for a period of time, from which it can be seen that the position of the blood sample remains basically ...

Embodiment 2

[0026] The solute transport electrospray ionization source loads the analytical sample in the middle of the solvent

[0027] At room temperature, inject 20 μL of solvent (such as methanol) into a capillary with a tip pore size of 20 μm, and then use a pipette to load 2 μL of blood sample containing the target drug compound in the middle of the solvent. When a DC voltage of 2.0kV is applied, the drug compound in the blood sample will be extracted by the solvent, and migrate to the tip of the capillary, and then the electrospray behavior will occur. In this process, the matrix of the blood sample will not change with the migration of the solvent. The specific process is as follows: figure 2 shown. figure 2 A is the situation of the electrospray capillary after adding 2 μL blood sample; figure 2 B is the picture taken by the capillary after applying a 2.0kV DC voltage to the spray capillary for a period of time, from which it can be seen that the position of the blood sample...

Embodiment 3

[0029] Effect of capillary tip size on solute transport electrospray ionization source

[0030] At room temperature, inject 20 μL of solvent (such as methanol) into capillaries with tip pore size of 1 μm and 20 μm, respectively, and then pipette 2 μL containing target drug compound such as verapamil (concentration of 1 μg·mL -1 ) of the blood sample was loaded at the end of the solvent. When a DC voltage of 2.0kV is applied, the solvent will be electrosprayed at the tip of the capillary. In this process, using a capillary with a tip size of 1 μm, no obvious changes in the intensity of characteristic molecular peaks were observed within 30 minutes (such as image 3 Shown in A); On the contrary, when using a capillary with a tip size of 20 μm, after about 2 minutes of solute migration, the mass spectrum signal of the target drug compound reaches the highest value (eg image 3 shown in B). It can be seen from this that since the position of the blood sample remains basically u...

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Abstract

The invention discloses a biological sample spectral analysis method based on a solute migration electrospray ionization technique. The biological sample spectral analysis method comprises the following steps: 1, under the conditions of normal temperature and normal pressure, firstly, injecting a solvent into a nanolitre spraying capillary tube; 2, loading a sample at the tail end of a solvent or in the middle of the solvent, applying direct-current voltage to the nanolitre spraying capillary tube so as to dissolve a target compound in the sample into the loaded solvent and migrate to the top end of the nanolitre spraying capillary tube, and furthermore performing electric spraying and detecting corresponding signals by using a mass spectrometry detector, thereby obtaining analysis result. The biological sample spectral analysis method has the characteristics that the operation is simple and convenient, no sample pretreatment is needed, the amount of the sample is small, the analysis sensitivity is high, real-time rapid analysis on the target compound is achieved, and the like.

Description

technical field [0001] The invention relates to a novel mass spectrometry ionization source technology, in particular to a biological sample mass spectrometry analysis method based on solute migration electrospray ionization technology. technical background [0002] Mass spectrometry has been widely used in the qualitative and quantitative analysis of target compounds in various fields due to its high sensitivity, less sample usage, fast analysis speed, and strong specificity. In order to further improve the sensitivity of mass spectrometry analysis and reduce the detection limit of analysis, complex samples usually need to be pretreated through tedious steps such as sample separation and purification before analysis. This process is not only time-consuming and labor-intensive, but also not conducive to small-volume biological samples. qualitative and quantitative analysis. [0003] In order to simplify the analysis process of complex samples, since Professor Fenn of Yale U...

Claims

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Application Information

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IPC IPC(8): G01N27/64
Inventor 张智平郑亚君张新荣
Owner XI'AN PETROLEUM UNIVERSITY
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