Dendrobium fimbriatum seed tissue culture and rapid propagation method
A Dendrobium fringe tissue culture technology, applied in the field of rapid propagation of Dendrobium fringe seed tissue culture, can solve problems such as artificial planting, and achieve the effects of saving energy, saving money, and improving tissue culture efficiency
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Embodiment 1
[0027] The invention discloses a rapid propagation method through tissue culture of Dendrobium fringe seeds. The seeds of Dendrobium tassels are taken as materials for in vitro induction culture to obtain tissue culture seedlings of Dendrobium tassels.
[0028] In the described Dendrobium fringe seed tissue culture rapid propagation method, comprise the following steps:
[0029] Step 1. Soak the fruits of Dendrobium tassels in 80% ethanol solution for 20 seconds, sterilize them with mercury chloride for 10 minutes, cut the fruits of Dendrobium tassels after cleaning to obtain pretreated seeds of Dendrobium tassels, and sow the pretreated seeds of Dendrobium tassels in On the induction medium, the cultivation temperature was 28°C, the light intensity was 2600 lux, and the light time was 15h / d, and cultured for 10 days to obtain small buds of Dendrobium nodii, wherein the induction medium was: MS-H Add 2.0 mg / L indole acetic acid, 2.0 mg / L naphthalene acetic acid, 0.5 mg / L thidi...
Embodiment 2
[0034] The invention discloses a rapid propagation method through tissue culture of Dendrobium fringe seeds. The seeds of Dendrobium tassels are taken as materials for in vitro induction culture to obtain tissue culture seedlings of Dendrobium tassels.
[0035] In the described Dendrobium fringe seed tissue culture rapid propagation method, comprise the following steps:
[0036] Step 1. Soak the fruits of Dendrobium tassels in 80% ethanol solution for 30 seconds, disinfect them with mercuric chloride for 20 minutes, cut the fruits of Dendrobium tassels after cleaning to obtain pretreated seeds of Dendrobium tassels, and sow the pretreated seeds of Dendrobium tassels in On the induction medium, cultured for 15 days under the condition that the culture temperature was 30°C, the light intensity was 2800 lux, and the light time was 14h / d, and the small shoots of Dendrobium fringe were obtained, wherein the induction medium was: MS-H Add 1.0 mg / L indole acetic acid, 3.0 mg / L naphth...
Embodiment 3
[0041] The invention discloses a rapid propagation method through tissue culture of Dendrobium fringe seeds. The seeds of Dendrobium tassels are taken as materials for in vitro induction culture to obtain tissue culture seedlings of Dendrobium tassels.
[0042] In the described Dendrobium fringe seed tissue culture rapid propagation method, comprise the following steps:
[0043] Step 1. Soak the fruits of Dendrobium tassels in 85% ethanol solution for 40 seconds, disinfect them with mercuric chloride for 20 minutes, cut the fruits of Dendrobium tassels after cleaning to obtain pretreated seeds of Dendrobium tassels, and sow the pretreated seeds of Dendrobium tassels in On the induction medium, cultured for 14 days at a culture temperature of 32°C, a light intensity of 3000 lux, and a light time of 13 h / d to obtain small buds of Dendrobium fringe, wherein the induction medium is: MS-H Basic medium, add 3.0mg / L indoleacetic acid, 4.0mg / L naphthaleneacetic acid, 1.0mg / L thidiazur...
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