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Extraction method of polysaccharides in coriolus versicolor

An extraction method and polysaccharide technology, which are applied in the field of bioengineering, can solve the problems of low polysaccharide extraction rate and complicated operation, and achieve the effects of high extraction rate, sufficient polysaccharide extraction and simple operation.

Inactive Publication Date: 2014-12-24
中山安荞生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional extraction methods of intracellular glycopeptides (polysaccharides) of Yunzhi are ultrasonic method and high-pressure homogenization method, but the above methods have disadvantages such as cumbersome operation or low extraction rate of polysaccharides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for extracting polysaccharides in Yunzhi, comprising the following steps:

[0027] (1) Sample pretreatment: take 3g of Versicolor versicolor, freeze-dry to constant weight, crush through a 40-mesh sieve, and take the sample powder under the sieve for later use;

[0028] (2) Cultivation and activation of strains: take the sample powder prepared in step (1) and place it in a slant medium for cultivation at a cultivation temperature of 25°C and a cultivation time of 3 days to obtain activated strains;

[0029] (3) Shake flask fermentation culture: Take the activated strain in step (2) and put it into a fermentation bottle for shake flask fermentation culture. The shaker speed is 200r / min, the culture temperature is 25°C, and the culture time is 3 days to obtain the strain fermentation liquid;

[0030] (4) Polysaccharide extraction: add the same amount of distilled water to further dissolve and dilute the fermentation broth of the strain obtained in step (3), put i...

Embodiment 2

[0037] A method for extracting polysaccharides in Yunzhi, comprising the following steps:

[0038] (1) Sample pretreatment: take 5g of Versicolor versicolor, freeze-dry to constant weight, crush through an 80-mesh sieve, and take the sample powder under the sieve for later use;

[0039] (2) Bacterial strain culture and activation: Take the sample powder prepared in step (1) and put it into the slant medium for cultivation, the cultivation temperature is 28°C, and the cultivation time is 5 days to obtain the activated strain;

[0040] (3) Shake flask fermentation culture: Take the activated strain in step (2) and put it into a fermentation bottle for shake flask fermentation culture. The shaker speed is 220r / min, the culture temperature is 28°C, and the culture time is 5 days to obtain the strain fermentation liquid;

[0041] (4) Polysaccharide extraction: Add the same amount of distilled water to the fermentation broth of the strain prepared in step (3) to further dissolve and...

Embodiment 3

[0048] A method for extracting polysaccharides in Yunzhi, comprising the following steps:

[0049] (1) Sample pretreatment: Take 4g of Versicolor versicolor, freeze-dry to constant weight, crush through a 60-mesh sieve, and take the sample powder under the sieve for later use;

[0050] (2) Bacterial strain culture and activation: Take the sample powder prepared in step (1) and put it into the slant medium for cultivation, the cultivation temperature is 26°C, and the cultivation time is 4 days to obtain the activated strain;

[0051](3) Shake flask fermentation culture: Take the activated strain in step (2) and put it into a fermentation bottle for shake flask fermentation culture. The shaker speed is 210r / min, the culture temperature is 26°C, and the culture time is 4 days to obtain the strain fermentation liquid;

[0052] (4) Polysaccharide extraction: add the same amount of distilled water to the fermentation broth of the strain prepared in step (3) to further dissolve and d...

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PUM

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Abstract

The invention relates to an extraction method of polysaccharides in coriolus versicolor. The method comprises the steps as follows: freezing and drying a proper amount of coriolus versicolor to the constant weight, and crushing and sieving the coriolus versicolor; placing the sieved sample powder into a slant culture medium for culture; placing activated strains into a fermentation flask for shaking flask fermentation culture to obtain a strain fermentation liquid; adding the same amount of distilled water into the strain fermentation liquid for dissolution and dilution, performing multiple times of microwave extraction, performing centrifuging, taking supernatant for rotary evaporation, adding absolute ethyl alcohol, performing ultrasonic even mixing, placing a mixture in a refrigerator of 4 DEG C overnight, performing centrifuging, discarding the supernatant, drying sediment to the constant weight, and obtaining the polysaccharides in the coriolus versicolor. According to the microwave extraction method adopted in the extraction method, extraction conditions are strictly optimized, the extraction time is in a range of 20-30 s, the extraction power is in a range of 260-265 W, the extraction temperature is in a range of 55-60 DEG C, and the number of extraction times is in a range of 3-5, so that the polysaccharides in the coriolus versicolor can be fully extracted, the extraction ratio is highest, meanwhile, the polysaccharides in the coriolus versicolor are prevented from being damaged, and compared with traditional methods, the extraction method is simpler to operate and short in operation time.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for extracting polysaccharides from versicolor versicolor. Background technique [0002] Coriolus versicolor (Coriolus versicolor), also known as Coriolus versicolor, Melaleuca versicolor, etc., belongs to Fungi, Basidiomycotina, Polyporaceae, and Coriolus. Coriolus versicolor is sweet and mild in taste, slightly cold in nature, has the effects of invigorating the spleen and dampness, relieving cough and asthma, clearing heat and detoxification, and anti-tumor. It is a traditional precious medicinal material in my country. Yunzhi polysaccharide is the main active ingredient of Yunzhi, which can activate immune cells, improve the body's immune function, anti-tumor, lower blood fat and antibacterial. In addition, Yunzhi polysaccharide has significant effects on learning and memory impairment caused by chronic stress. Improve the effect, therefore, Yunzhi...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 刘巴宁郑安乔
Owner 中山安荞生物科技有限公司
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