Tissue culture seedling and culturing method for fir wood of excellent clonal 11C

A tissue culture and clonal technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of sub-generation multiplication, rooting rate, low survival rate of transplanting, no method of cultivating seedlings, etc., and achieve rooting rate. High, effective seedling rate, high multiplication effect

Inactive Publication Date: 2014-11-19
FUJIAN ACAD OF FORESTRY
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Problems solved by technology

The techniques and patents related to the tissue culture of Chinese fir clones have been publicly reported. Due to the differences in the growth habits of different Chinese fir individuals, the technical methods of different stages of tissue culture of different Chinese fir clones cannot be simply copied and applied mechanically.
The tissue culture method for growing seedlings of Chinese fir clones in the prior art is not suitable for the cultivation of the fine Chinese fir clone 11C, which is manifested in the low subculture multiplication rate, rooting rate and transplanting survival rate of the fine Chinese fir clone 11C.
In the prior art, there is no method for raising seedlings by tissue culture for the excellent Chinese fir clone 11C

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  • Tissue culture seedling and culturing method for fir wood of excellent clonal 11C
  • Tissue culture seedling and culturing method for fir wood of excellent clonal 11C
  • Tissue culture seedling and culturing method for fir wood of excellent clonal 11C

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Embodiment Construction

[0014] In order to describe in detail the technical content, structural features, achieved objectives and effects of the present invention, the following will be described in detail in conjunction with the embodiments.

[0015] The most critical idea of ​​the present invention is: through the design of induction medium, subculture medium and rooting medium formula, make the tissue culture seedling raising method of fine Chinese fir clone 11C of the present invention have high induction rate, high multiplication multiple and rooting rate high merit.

[0016] The method for raising seedlings by tissue culture of a Chinese fir fine clone 11C provided by the invention comprises the following steps:

[0017] (1) Insert the explants of the fine Chinese fir clone 11C into the induction medium, and carry out the adventitious bud induction culture, and the formula of the induction medium includes the following components: 1 / 2MS, 0.5-0.8mg / L of 6- BA, 0.1-0.2mg / L IBA, 30g / L sucrose and...

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Abstract

The invention relates to a tissue culture seedling culturing method for fir wood of excellent clonal 11C. The method comprises the following steps:(1) the explant of the fir wood of the excellent clonal 11C is innoculated in an inducing culture medium, the inducing culture of an indefinite bud is performed, and the indefinite bud is obtained; (2) the indefinite bud is innoculated into a subculture proliferation culture medium, the subculture proliferation culture is performed, and a subcultured seedling is obtained; (3) the subcultured seedling is innoculated into a rooting culture medium, inducing rooting culture is performed, and a rooting seedling is obtained. The tissue culture seedling culturing method for the fir wood of the excellent clonal 11C, disclosed by the invention, has the advantages of high inductivity, high proliferation rate, high effective seedling rate and high rooting rate.

Description

technical field [0001] The invention relates to the field of plant tissue culture and seedling cultivation, in particular to a method for tissue culture and seedling cultivation of an excellent Chinese fir clone 11C. Background technique [0002] The fine Chinese fir clone 11C is an excellent Chinese fir clone bred by Zheng Renhua of the Fujian Academy of Forestry Sciences. It has the excellent characteristics of fast growth and high quality. The techniques and patents related to the tissue culture of excellent Chinese fir clones have been reported publicly. Due to the differences in the growth habits of different Chinese fir individuals, the technical methods at each stage of tissue culture of different Chinese fir clones cannot be simply copied and applied mechanically. The tissue culture seedling raising method of the Chinese fir clone in the prior art is not suitable for the cultivation of the excellent Chinese fir clone 11C, which is manifested in that the cultured Chin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 郑仁华肖晖苏顺德方禄明谢汝根黄金华
Owner FUJIAN ACAD OF FORESTRY
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